TALENs Methods and Protocols
This volume provides a comprehensive collection of protocols on new technology across various model organisms. Chapters describe species-specific methods to generate new mutants the content is completed by chapters on natural TAL effectors, TAL elem
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Ralf Kühn Wolfgang Wurst Benedikt Wefers Editors
TALENs Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
TALENs Methods and Protocols Edited by
Ralf Kühn Max-Delbrück-Center for Molecular Medicine, Berlin, Germany; Berlin Institute of Health, Berlin, Germany
Wolfgang Wurst Institute of Developmental Genetics, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany; Technische Universität München, Freising-Weihenstephan, Germany; Deutsches Zentrum für Neurodegenerative Erkrankungen e. V. (DZNE), Munich, Germany; Munich Cluster for Systems Neurology (SyNergy), Ludwig-Maximilians-Universität München, Munich, Germany
Benedikt Wefers Institute of Developmental Genetics, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany; Deutsches Zentrum für Neurodegenerative Erkrankungen e. V. (DZNE), Munich, Germany
Editors Ralf Kühn Max-Delbrück-Center for Molecular Medicine Berlin, Germany Berlin Institute of Health, Berlin, Germany Benedikt Wefers Institute of Developmental Genetics Helmholtz Zentrum München German Research Center for Environmental Health Neuherberg, Germany Deutsches Zentrum für Neurodegenerative Erkrankungen e. V. (DZNE) Munich, Germany
Wolfgang Wurst Institute of Developmental Genetics Helmholtz Zentrum München German Research Center for Environmental Health Neuherberg, Germany Technische Universität München Freising-Weihenstephan, Germany Deutsches Zentrum für Neurodegenerative Erkrankungen e. V. (DZNE) Munich, Germany Munich Cluster for Systems Neurology (SyNergy) Ludwig-Maximilians-Universität München Munich, Germany
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-2931-3 ISBN 978-1-4939-2932-0 (eBook) DOI 10.1007/978-1-4939-2932-0 Library of Congress Control Number: 2015949823 Springer New York Heidelberg Dordrecht London © Springer Science+Business Media New York 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the pub
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