The assessment of electromagnetic fields-caused genetic diversity of Tea Camellia sinensis L. using ISSR and SCoT marker
- PDF / 2,465,250 Bytes
- 14 Pages / 595.276 x 790.866 pts Page_size
- 22 Downloads / 198 Views
GENETICS & EVOLUTIONARY BIOLOGY - ORIGINAL ARTICLE
The assessment of electromagnetic fields‑caused genetic diversity of Tea Camellia sinensis L. using ISSR and SCoT markers Seyed Mohammad Yousef Azizi1 · Siavash Hosseini Sarghein1 · Ahmad Majd2 · Maryam Peyvandi2 Received: 17 February 2020 / Revised: 31 August 2020 / Accepted: 1 September 2020 © Botanical Society of Sao Paulo 2020
Abstract The effects of electromagnetic fields on plants have been studied during the last decades. The electromagnetic fields as abiotic stress can induce genetic variation in living organisms including plants by different mechanisms. The aim of the present study is to assay the possible electromagnetic fields-caused genetic diversity of Tea Camellia sinensis L. which is known as favorite herbal beverages by many biochemical constituents. To do so, intersimple sequence repeats (ISSR) and start codon targeted (SCoT) loci were used as molecular markers. Different electromagnetic field treatments by 1, 2, 4 and 6 mT of intensity, for 30 and 60 min per day, for 7 continuous days were imposed, following by DNA extraction and PCR amplification. AMOVA, PCoA and STRUCTURE analysis based on the ISSR and SCoT markers showed that regardless of the EMFs intensity, 30 min of radiation causes more genetic variation than that of 60 min does until the intensity was increased up to 6 mT, due to direct and indirect mechanisms such as guanine modification, methylation of cytosines, Fenton reaction and direct DNA strands breaking. ISSR sequences, however, underwent more variation than SCoT. Keywords Electromagnetic fields · Genetic variation · ISSR and SCoT · Molecular markers · Tea Camellia sinensis L.
1 Introduction The specie Camellia sinensis L. (Tea) is one of the most favorite herbal beverages throughout the world, originated in China (Del Rio et al. 2004). The shoot part of tea contains many biochemical constituents such as phenolic components, alkaloids, vitamins and enzymes. These natural substances are helpful for treating some of the human diseases (Samynathan et al. 2016). Although tea is mostly drunk for amusement, the medical benefits of this plant have been historically investigated since thousands of years ago. * Seyed Mohammad Yousef Azizi [email protected] Siavash Hosseini Sarghein [email protected] Ahmad Majd a_majd@iau‑tnb.ac.ir Maryam Peyvandi [email protected] 1
Department of Biology, Faculty of Science, Urmia University, Urmia, Iran
Biology Department, Faculty of Bio Sciences, Tehran North Branch, Islamic Azad University, Tehran, Iran
2
Camellia sinensis L. belongs to Theaceae family and has two main varieties named: C. sinensis (var. sinensis), by smallleaved and bush-like shape, distributed in Southeast of Asia; and C. sinensis (var. assamica), by large-leaved and trees like, growing in India and semi-tropical climate (Mahmood et al. 2010). Tea is mostly used as a fermented type named black tea. The unfermented type however which is known as green tea has become more favorite during the last decades (
Data Loading...
