The complete mitochondrial genome of Sturisomatichthys panamense (Siluriformes: Loricariidae) analysed by next-generatio
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ORIGINAL ARTICLE
The complete mitochondrial genome of Sturisomatichthys panamense (Siluriformes: Loricariidae) analysed by next-generation sequencing and phylogeny of the catfish subfamily Loricariinae (Siluriformes: Loricariidae) Fang Ren 1,2 & Dunxue Chen 1,2 & Xiuhui Ma 1,2 Received: 5 May 2019 / Accepted: 21 November 2019 # Institute of Zoology, Slovak Academy of Sciences 2019
Abstract Sturisomatichthys panamense (Loricariidae, armored catfishes) is widespread throughout Central and South America from Pacific slope rivers of Panama to Caribbean slope rivers of Colombia. Due to the incredible body shape, it becomes one of the most popular pet fish in the world. However, little research has been done for this species, especially its genetic resource. In the present study, the complete mitochondrial DNA genome of S. panamense was first reported by next-generation sequencing method. The entire length of mitochondrial genome is 16,497 bp and the nucleotide composition was made up of 31.2% A, 28.3% C, 25.5% T, and 15.0% G, respectively, indicating an A + T (56.7%)-rich feature in the S. panamense complete mitogenome. The mitogenome includes 22 transfer RNA genes, 2 ribosomal RNA genes, 13 protein-coding genes and one control region. With the exception of 8 tRNA genes and NADH6, mitochondrial genes are encoded on the heavy strand, which was in line with other vertebrates. Phylogenetic trees centred on the Loricariinae were reconstructed using another 72 reported cytochrome c oxidase I (cox1) gene sequences. They indicated monophyly of the Loricariinae and presented a basal split within the Loricariinae resulting in two major tribes, the Loricariini and the Harttiini. Sturisomatichthys panamense was placed with Sturisoma panamense and formed a sister group to Sturisomatichthys citurensis in the phylogenetic trees. Keywords Mitochondrial genome . Sturisomatichthys panamense . Next-generation sequencing . Phylogeny
Introduction The Loricariinae species represent a highly diversified subfamily among the large Neotropical catfish family Loricariidae, or suckermouth armored catfish, which are distributed in Costa Rica, Panama and South America (Nelson 2006). The Loricariidae is the largest family of catfishes, with eight subfamilies, 116 genera and 980 valid species in this family (Fricke et al. 2019). To date, however, only three complete mtDNA genomes have been available (Sato et al. 2018).
* Xiuhui Ma [email protected] 1
School of Animal Science, Guizhou University, Huaxi district, Guiyang 550025, Guizhou Province, China
2
Key Laboratory of Animal Genetics, Breeding and Reproduction in The Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang, China
Among loricariids, members of the Loricariinae are united by a long and flattened caudal peduncle and the absence of an adipose fin. They live stuck to the substrate and show marked variations in body shape according to the various habitats colonized, from lotic to lentic systems, on mineral or organic substrates (Covain and Fisch-Muller 2007).
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