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TWO Co(II)-BASED COORDINATION POLYMERS: STRUCTURAL INSIGHTS AND STIMULATION ACTIVITY ON OSTEOBLAST DIFFERENTIATION BY INCREASING THE WNT/β-CATENIN SIGNALING PATHWAY ACTIVATION L. Shi1, J. Feng2, J. Zhang2, and Q. Wang3*

In this work, two Co(II)-based coordination polymers with the chemical formulae [CoCl(cptpy)⋅2H2O]n (1) and [Co2Cl2(cptpy)2(ma)H2O]n (2, Hcptpy = 4′-(4-carboxyphenyl)-2,2′:6′,2″-terpyridine, ma = melamine) are hydrothermally synthesized without or with employment of the template melamine. The as-prepared two complexes are studied via single crystal X-ray diffraction along with the elemental analysis. Furthermore, the protective ability of the compounds on the osteoblast differentiation is evaluated and the related detailed mechanism is explored. Firstly, the Western blot is used to detect the activation of the Wnt/β-catenin signaling pathway after treatment with the compounds. Next, the RT-PCR assay is used to measure the relative expression of indicators of vegf and vwf angiogenesis. DOI: 10.1134/S0022476620090140 Keywords: coordination polymer, Co(II) complex, osteoblast differentiation, Wnt/β-catenin.

INTRODUCTION A recent report has indicated that about 3-10% of patients have delayed healing and nonunion after fracture, therefore, it is urgent to find new synthetic candidates to promote bone regeneration and repair and improve the treatment efficacy of fractures [1]. In the past decades, more and more researches revealed that multiple signaling inflammatory cytokine pathways participate in the regulation of osteoblast differentiation. Among these signaling pathways, the Wnt/βcatenin signaling pathway was regarded as playing the most important role in skeletal development and bone tissue homeostasis [2, 3]. The increased or decreased level of β-catenin could affect fracture healing at the early stages of fracture. In the late stage of fracture, when bone marrow mesenchymal stem cells have differentiated into osteoblasts, β-catenin can also promote osteoblast proliferation and post-fracture healing [4, 5]. Functional coordination polymers (CPs) constructed from transition metal ions with unpaired electrons have been paid close attention by numerous chemists, not only because of their novel and polytropic architectures, but also because of

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Department of Joint Surgery, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, People′s Republic of China. 2Nursing Department, 986 Hospital of Air Force of PLA, Xi′an, Shaanxi, People′s Republic of China. 3Orthopaedic Operating Room, Xijing Hospital, AirForce Medical University, Xi′an, Shaanxi, People′s Republic of China; *[email protected]. Original article submitted December 2, 2019; revised Februiary 22, 2020; accepted March 6, 2020. 0022-4766/20/6109-1461 © 2020 by Pleiades Publishing, Ltd.

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their characteristic properties [6-9]. Up to now, preliminary achievements have been made in the controllable constructions of this kind of CPs via regulating the synthetic conditions to build different secondary building units