The Golgi Complex Methods and Protocols
Focusing on new methods and techniques developed to address longstanding questions about the Golgi complex, this volume explores a diverse set of chapters, ranging from live and fixed cell imaging techniques toin vitro biochemical reconstitution syst
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William J. Brown Editor
The Golgi Complex Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
The Golgi Complex Methods and Protocols
Edited by
William J. Brown Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY, USA
Editor William J. Brown Department of Molecular Biology and Genetics Cornell University Ithaca, NY, USA
Videos can also be accessed at http://link.springer.com/book/10.1007/978-1-4939-6463-5_1. ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6461-1 ISBN 978-1-4939-6463-5 (eBook) DOI 10.1007/978-1-4939-6463-5 Library of Congress Control Number: 2016948830 © Springer Science+Business Media New York 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC New York
Preface The Golgi complex has fascinated cell biologists for decades because of its unique architecture and role as a “Grand Central Station” for trafficking soluble and membranous cargo through the secretory and endocytic pathways. Indeed, the Golgi’s unusual architecture itself has thwarted efforts to fully understand how secretory cargo is imported, passed through, and exported from the Golgi complex. Nevertheless, tremendous progress has been made to reveal the molecular mechanism underlying the functional organization of the Golgi complex. Accordingly, new methods and techniques have been developed to address long-standing questions about the Golgi complex, which are the focus of this book. The chapters herein encompass a diverse set of methods for studying the Golgi complex, ranging from live and fixed cell imaging techniques to in vitro biochemical reconstitution systems. The book is targeted t
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