The BAM Complex Methods and Protocols
This volume is comprised of a collection of experimental protocols for common techniques and strategies used to study the biogenesis of b-barrel outer membrane proteins in Gram-negative bacteria. The BAM Complex: Methods and Protocols guides readers throu
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Susan K. Buchanan Nicholas Noinaj Editors
The BAM Complex Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
The BAM Complex Methods and Protocols
Edited by
Susan K. Buchanan Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA
Nicholas Noinaj Department of Biological Sciences, Markey Center for Structural Biology, Purdue University, West Lafayette, IN, USA
Editors Susan K. Buchanan Laboratory of Molecular Biology National Institute of Diabetes and Digestive and Kidney Diseases National Institutes of Health Bethesda, MD, USA
Nicholas Noinaj Department of Biological Sciences Markey Center for Structural Biology Purdue University, West Lafayette IN, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-2870-5 ISBN 978-1-4939-2871-2 (eBook) DOI 10.1007/978-1-4939-2871-2 Library of Congress Control Number: 2015949089 Springer New York Heidelberg Dordrecht London © Springer Science+Business Media New York 2015 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)
Preface Cells are encapsulated by a single lipid bilayer called a membrane that forms the boundary separating the inside of the cell from the outside. The membrane serves many essential functions for the cell including nutrient import, signaling, motility, adhesion, endocytosis, and replication. These functions are accomplished by a large family of proteins called membrane proteins that are either partially or fully integrated into the membrane. Fully integrated membrane proteins are embedded into the membrane by hydrophobic do
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