Description of Paenibacillus yunnanensis sp. nov., Isolated from a Tepid Spring
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Description of Paenibacillus yunnanensis sp. nov., Isolated from a Tepid Spring Manik Prabhu Narsing Rao1,2 · Zhou‑Yan Dong1 · S. Amoolya2 · Srujana Neelavar2 · Bing‑Bing Liu3 · Shu‑Xian Guo3 · Wael Hozzein4,5 · Wen‑Jun Li1 Received: 22 March 2020 / Accepted: 8 June 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract A Gram stain-positive, rod-shaped and motile bacterium, designated SYSU G01003T was isolated from a sediment sample collected from tepid spring in Tengchong, Yunnan province, southwestern China. Growth observed at temperature ranging 28–37 °C (optimum 37 °C) and pH 6.0–8.0 (optimum pH 7.0). Tolerance to NaCl was up to 2.5% (w/v) (optimal in the absence of NaCl). The cell wall peptidoglycan is meso-2,6-diaminopimelic acid and MK-7 as the only respiratory quinone. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and unidentified aminophospholipid, phospholipid, and polar lipid. The major fatty acids are C16:0, anteiso-C15:0 and C14:0. The genomic DNA G + C content of the type strain was 54.0 mol%. The average nucleotide identity (ANIb and ANIm) values between SYSU G01003T and Paenibacillus azotifigens LMG 29963T were below the cut-off level (95–96%) recommended as the average nucleotide identity (ANI) criterion for interspecies identity. Based on the above results strain SYSU G 01003T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus yunnanensis sp. nov. is proposed. The type strain, SYSU G01003T (=KCTC 43132T = CGMCC 1.17384T).
Introduction
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00284-020-02087-z) contains supplementary material, which is available to authorized users. * Wen‑Jun Li [email protected] 1
State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, China
2
Department of Biotechnology, Dayananda Sagar College of Engineering (Affiliated To Visvesvaraya Technological University, Belagavi), , Bangalore, Karnataka 560111, India
3
Henan Key Laboratory of Industrial Microbial Resources and Fermentation Technology, College of Biological and Chemical Engineering, Nanyang Institute of Technology, Nanyang 473004, China
4
Zoology Department, College of Science, King Saud University, Riyadh 11451, Saudi Arabia
5
Botany and Microbiology Department, Faculty of Science, Beni-Suef University, Beni Suef 65211, Egypt
Traditionally, any bacterium was classified as a Bacillus, that was rod-shaped, aerobic, or facultatively anaerobic, and form endospores [1]. Based on the traditional classification, species of Paenibacillus were originally included in the genus Bacillus, but based on 16S rRNA gene sequence analysis the genus Bacillus was split into several genera and Paenibacillus was one among them [2, 3]. The genus Paenibacillus was proposed by Ash et al. [3] with Paenibacillus polymyxa as type species. At
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