Paracoccus zhejiangensis sp. nov., isolated from activated sludge in wastewater-treatment system
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ORIGINAL PAPER
Paracoccus zhejiangensis sp. nov., isolated from activated sludge in wastewater-treatment system Zhi-Guo Wu • Dao-Feng Zhang • Ya-Long Liu Fang Wang • Xin Jiang • Chao Li • Shun-Peng Li • Qing Hong • Wen-Jun Li
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Received: 6 February 2013 / Accepted: 2 May 2013 / Published online: 8 May 2013 Ó Springer Science+Business Media Dordrecht 2013
Abstract A bacterial strain, designated J6T, was isolated from activated sludge, collected from a chemical wastewater treatment system in Zhejiang Province of China. The cells stained Gram-negative, were aerobic, pale-yellow, and non-motile short rods. Phylogenetic analysis of the 16S rRNA gene sequence indicated that the closest relative of this organism was Paracoccus aminophilus KACC 12262T = JCM 7686T (97.4 % sequence similarity). Strain J6T grew at 10–37 °C (optimum 30 °C), at pH 6.0–8.0 (optimum pH 7.0) and with 0–5 % NaCl (optimum 3 %, w/v). The predominant cellular fatty acid found was summed feature 8(C18:1 x7c and/or C18:1 x6c; 82.8 %). The major respiratory quinone-detected
Zhi-Guo Wu and Dao-Feng Zhang contributed equally to this study.
Electronic supplementary material The online version of this article (doi:10.1007/s10482-013-9932-2) contains supplementary material, which is available to authorized users. Z.-G. Wu Y.-L. Liu C. Li S.-P. Li Q. Hong (&) Key Lab of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, Jiangsu, People’s Republic of China e-mail: [email protected]
was Q-10 and the DNA G?C content was 61.9 mol %. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine and several unknown polar lipids. Strain J6T showed low DNA–DNA relatedness values with P. aminophilus KACC 12262T (28 ± 3 %). The phylogenetic analysis, DNA–DNA hybridization, whole-cell fatty acid composition as well as biochemical characteristics allowed clear differentiation of the isolate from the other type strains of already described Paracoccus species. It is evident from the genotypic, phenotypic and chemotaxonomic analyses that strain J6T should be classified as a novel species of the genus Paracoccus, for which the name P. zhejiangensis sp. nov. is proposed. The type strain is J6T (KACC 16703T = CCTCC AB 2012031T). Keywords Paracoccus zhejiangensis sp. nov. Polyphasic taxonomy 16S rRNA
D.-F. Zhang W.-J. Li (&) The Key Laboratory for Microbial Resources of the Ministry of Education and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, Yunnan, People’s Republic of China e-mail: [email protected]
Z.-G. Wu F. Wang X. Jiang Q. Hong State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, People’s Republic of China
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Introduction The genus Paracoccus belongs to the Alphaproteobacteria and was proposed by Davis et al. (1969)
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