Diaphorobacter ruginosibacter sp. nov., isolated from soybean root nodule, and emended description of the genus Diaphoro
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ORIGINAL PAPER
Diaphorobacter ruginosibacter sp. nov., isolated from soybean root nodule, and emended description of the genus Diaphorobacter Xiu Li Wei1 · Meng Sha Han1 · Cong Cong Xia1 · Shu Lan Ding1 · Lin Xu1 · Yan Bing Lin1 · Li Wang1 · Ge Hong Wei1
Received: 22 October 2014 / Revised: 1 March 2015 / Accepted: 3 March 2015 © Springer-Verlag Berlin Heidelberg 2015
Abstract A gram-negative bacterium designated BN30T, which is motile with a polar flagellum, non-endospores forming, oxidase- and catalase-positive, was isolated from soybean root nodule. The organism is facultative anaerobic and surface-wrinkled rod. It can grow at 10–40 °C, pH 6–8 and 6 % (w/v) NaCl. BLASTn search based on 16S rRNA gene sequence revealed that the strain is closely related to Diaphorobacter aerolatus 8604S-37T, Alicycliphilus denitrificans K601T, Simplicispira limi EMB325T, Diaphorobacter nitroreducens NA10BT and Diaphorobacter oryzae RF3T, which all belonged to the family Comamonadaceae in class Betaproteobacteria. Phylogenetic analysis showed that the strain formed a firm clade with three Diaphorobacter species, being closest to Diaphorobacter aerolatus 8604S-37T
with similarity of 98.64 %. DNA–DNA relatedness values between strain BN30T and five reference strains ranged from 11.5 to 35.9 %. All the results of phylogenetic analysis, chemotaxonomical data (predominant fatty acids are C16:0, sum feature 3, sum feature 8 and C17:0 cyclo; major polar lipids are diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol; major quinone is Q-8; G+C of total DNA is 65.2 %), physiological and phenotypic results supported that BN30T represented a novel species within the genus Diaphorobacter. The name Diaphorobacter ruginosibacter was proposed, and the type strain is BN30T (=ACCC06116T = DSM 27467T). Keywords Diaphorobacter ruginosibacter · Soybean · Root nodule · Endophytic
Communicated by Erko Stackebrandt. The GenBank accession number for the 16S rRNA gene sequence of strain BN30T is KC825337. The strain had been deposited in Leibniz-Institut Deutsche Sammlung von Mikroorganismen und Zellkulturen Gmbh (DSMZ) and Agricultural Culture Collection of China (ACCC) under deposition number DSM27467 and ACCC06116. Reference strains Diaphorobacter nitroreducens NA10BT, Diaphorobacter oryzae RF3T, Simplicispira limi EMB325T and Alicycliphilus denitrificans K601T were bought from DSMZ; Diaphorobacter aerolatus 8604S-37T was supplied kindly by Professor Kwon (National Academy of Agricultural Science, Republic of Korea). Electronic supplementary material The online version of this article (doi:10.1007/s00203-015-1102-7) contains supplementary material, which is available to authorized users. * Ge Hong Wei [email protected] 1
State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, College of Life Sciences, Northwest A&F University, Yangling 712100, Shaanxi, China
Introduction Root nodules are complicated symbionts formed by rhizobia and legume plants. Recent studies revealed that not only rhizob
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