Rhizobium qilianshanense sp. nov., a novel species isolated from root nodule of Oxytropis ochrocephala Bunge in China
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ORIGINAL PAPER
Rhizobium qilianshanense sp. nov., a novel species isolated from root nodule of Oxytropis ochrocephala Bunge in China Lin Xu • Yong Zhang • Zheng Shan Deng Liang Zhao • Xiu Li Wei • Ge Hong Wei
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Received: 12 June 2012 / Accepted: 22 October 2012 Ó Springer Science+Business Media Dordrecht 2012
Abstract During a study of the diversity and phylogeny of rhizobia isolated from root nodules of Oxytropis ochrocephala grown in the northwest of China, four strains were classified in the genus Rhizobium on the basis of their 16S rRNA gene sequences. These strains have identical 16S rRNA gene sequences, which showed a mean similarity of 94.4 % with the most closely related species, Rhizobium oryzae. Analysis of recA and glnA sequences showed that these strains have less than 88.1 and 88.7 % similarity with the defined species of Rhizobium, respectively. The genetic diversity revealed by ERIC-PCR fingerprinting indicated that the isolates correspond to different strains. Strain CCNWQLS01T contains Q-10 as the predominant ubiquinone. The major fatty acids were identified as feature 8 (C18: 1x7c and/or C18: 1x6c; 67.2 %). Therefore, a novel species Rhizobium qilianshanense
Electronic supplementary material The online version of this article (doi:10.1007/s10482-012-9840-x) contains supplementary material, which is available to authorized users. L. Xu Z. S. Deng L. Zhao X. L. Wei G. H. Wei (&) State Key Laboratory of Soil Erosion and Dryland Farming On the Loess Plateau, College of Life Sciences, Northwest A&F University, Yangling 712100, Shaanxi, China e-mail: [email protected] L. Xu Y. Zhang Department of Life Science and Engineering, Hexi University, Zhangye 734000, Gansu, China
sp. nov. is proposed, and CCNWQLS01T (= ACCC 05747T = JCM 18337T) is designated as the type strain. Keywords Rhizobium Novel species Oxytropis ochrocephala China
Introduction Oxytropis ochrocephala are perennial wild plants belonging to the subfamily Faboideae of family Leguminosae, distributed in some regions of Gansu, Qinghai and Sichuan province and can be used as foliage, green manure, as well as preventing soil erosion (Allen and Allen 1981). In addition, the whole plant is being considered in Chinese herbal medicine for the treatment of clearing heat and detoxicating, activating blood and dissolving stasis (Liu et al. 2010). Despite the huge number of species within the family Leguminosae which contains about 18,000 species, only small fraction of rhizobia have been described up to date. The taxonomy of rhizobia is in a state of flux for numbers of revisions to its description and the addition of many novel species. They are currently classified into the genera Rhizobium, Ensifer, Allorhizobium, Mesorhizobium, Bradyrhizobium, Azorhizobium, Methylobacterium, Devosia, Ochrobactrum, Phyllobacterium and Shinella in Alphaproteobacteria (Sy et al. 2001; Wei et al. 2002) as well as Burkholderia and Cupriavidus within the beta subclass of proteobacteria (Moulin et al. 2001).
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