Georgenia faecalis sp. nov. isolated from the faeces of Tibetan antelope
- PDF / 756,081 Bytes
- 7 Pages / 612 x 792 pts (letter) Page_size
- 10 Downloads / 153 Views
eISSN 1976-3794 pISSN 1225-8873
Georgenia faecalis sp. nov. isolated from the faeces of Tibetan antelope§ Xiaoxia Wang1,2, Jing Yang2,3,4, Yuyuan Huang5, Xiaomin Wu6, Licheng Wang1, Limei Han1, Sha Li1, Huan Li1, Xiaoying Fu1, Hai Chen1, and Xiong Zhu1* 1
Central & Clinical Laboratory of Sanya People’s Hospital, Sanya, Hainan 572000, P. R. China State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing 102206, P. R. China 3 Shanghai Institute for Emerging and Re-emerging Infectious Diseases, Shanghai Public Health Clinical Center, Shanghai 201508, P. R. China 4 Research Units of Discovery of Unknown Bacteria and Function, Chinese Academy of Medical Sciences, Beijing 100730, P. R. China 5 Guangxi Key Laboratory of AIDS Prevention and Treatment & Guangxi Collaborative Innovation Center for Biomedicine, School of Public Health, Guangxi Medical University, Nanning 530021, Guangxi, P. R. China 6 Shaanxi Institute of Zoology, Xi′an 710032, P. R. China 2
(Received Feb 4, 2020 / Revised Jun 11, 2020 / Accepted Jun 16, 2020)
Two aerobic, Gram-stain-positive, non-motile, non-sporulating coccoid strains, designated ZLJ0423T and ZLJ0321, were isolated from the faeces of Tibetan antelope (Pantholops hodgsonii). Their optimal temperature, NaCl concentration and pH for growth were 28°C, 0.5% (w/v) NaCl and pH 7.5, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains ZLJ0423T and ZLJ0321 were very similar to each other (99.8%) and had a sequence similarity of 97.0% with Georgenia satyanarayanai NBRC 107612T and Georgenia subflava CGMCC 1.12782T. Phylogenomic analysis based on 688 core genes indicated that these strains formed a clade with G. satyanarayanai NBRC 107612T and Georgenia wutianyii Z294T. The predominant cellular fatty acids were anteiso-C15:0, anteiso-C15:1 A and C16:0. The major menaquinone was MK-8(H4). The cell-wall amino acids consisted of alanine, lysine, glycine and aspartic acid, with lysine as the diagnostic diamino acid. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides and two unidentified lipids formed the polar lipid profile. The DNA G + C content of both isolates was 73.9 mol%. The digital DNA–DNA hybridization value between strains ZLJ0423T and ZLJ0321 was 91.2%, but their *For correspondence. E-mail: [email protected] The GenBank/ EMBL/ DDBJ accession numbers for the 16S rRNA gene sequences of strains ZLJ0423T and ZLJ0321 are MH136554 and MK156345 respectively. The GenBank/ EMBL/ DDBJ accession numbers for the genome sequences of strains ZLJ0423T and ZLJ0321 are CP033325 and RHDW00000000, respectively. § Supplemental material for this article may be found at http://www.springerlink.com/content/120956. Copyright ⓒ 2020, The Microbiological Society of Korea
values with closely related species and other available type strains of the genus Georgenia were lower than the 70%
Data Loading...
