Novosphingobium endophyticum sp. nov. isolated from roots of Glycyrrhiza uralensis
- PDF / 560,787 Bytes
- 8 Pages / 595.276 x 790.866 pts Page_size
- 86 Downloads / 206 Views
ORIGINAL PAPER
Novosphingobium endophyticum sp. nov. isolated from roots of Glycyrrhiza uralensis Yan‑Qiong Li1,4 · Li Li2 · Wei Chen3 · Yan‑Qing Duan3 · Salam Nimaichand4,5 · Jian‑Wei Guo2,6 · Rui Gao3 · Wen‑Jun Li2,4,5
Received: 2 April 2015 / Revised: 7 May 2015 / Accepted: 19 May 2015 / Published online: 6 June 2015 © Springer-Verlag Berlin Heidelberg 2015
Abstract A novel Gram-staining-negative, non-motile, rod-shaped and aerobic bacterial strain, designated EGI 60015T, was isolated from healthy roots of Glycyrrhiza uralensis F. collected from Yili County, Xinjiang Province, Northwest China. The 16S rRNA gene sequence of strain EGI 60015T was found to show 97.6 % sequence similarity with Novosphingobium pentaromativorans US6-1T. The phylogenetic analysis based on 16S rRNA gene sequences showed that the strain formed a clade with N. pentaromativorans US6-1T in the neighbor-joining tree. Q-10 was identified as the respiratory quinone of strain EGI 60015T. The major fatty acids were summed feature 8 (C18:1 ω6c and/or C18:1 ω7c; 55.04 %), summed feature 4 (C17:1 anteiso B and/or iso I; 18.34 %) and summed feature 3 (C16:1 ω6c and/or C16:1 ω7c; 8.53 %). The polar lipids detected were phosphatidylethanolamine, Communicated by Erko Stackebrandt.
phosphatidylmethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine and sphingoglycolipids. The DNA G+C content of strain EGI 60015T was determined to be 66.6 mol%. The genomic DNA relatedness value between EGI 60015T and N. pentaromativorans US6-1T (54 %) was below the 70 % limit for species identification. Based on the result of the molecular studies supported by its morphological, physiological, chemotaxonomic and other differentiating phenotypic characteristics, strain EGI 60015T was considered to represent a novel species within the genus Novosphingobium, for which the name Novosphingobium endophyticum sp. nov. is proposed. The type strain is EGI 60015T (=CGMCC 1.15095T = KCTC 42486T = DSM 29948T). Keywords Novosphingobium endophyticum sp. nov. · Endophytic bacteria · Glycyrrhiza uralensis F.
Yan-Qiong Li, Li Li and Wei Chen have contributed equally to this work. Electronic supplementary material The online version of this article (doi:10.1007/s00203-015-1124-1) contains supplementary material, which is available to authorized users. * Rui Gao [email protected] * Wen‑Jun Li [email protected] 1
Kunming Medical University Haiyuan College, Kunming 650106, People’s Republic of China
2
Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürümqi 830011, People’s Republic of China
3
China Tobacco Yunnan Industrial Co. Ltd., Kunming 650231, People’s Republic of China
4
Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, People’s Republic of China
5
State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, College of Ecology and Evolution, Sun Yat-Sen University, Guangzhou 510275, People’s
Data Loading...
