Massilia aquatica sp. nov., Isolated from a Subtropical Stream in China
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Massilia aquatica sp. nov., Isolated from a Subtropical Stream in China Hui‑Bin Lu1,2,3 · Tong‑Chu Deng1,2,3 · Mei‑Ying Xu1,2,3 Received: 1 April 2020 / Accepted: 18 June 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract A Gram-stain-negative, catalase- and oxidase-positive, rod-shaped and motile strain FT127WT was isolated from a subtropical stream in China. Comparison based on 16S rRNA gene sequences showed that strain FT127WT belongs to genus Massilia and shares 98.5% similarity with Massilia buxea A9T as its closest neighbor. The genome size of strain F T127WT was 6.65 Mbp with G + C content of 65.3%. The calculated pairwise OrthoANIu values and digital DNA–DNA hybridization values between strain FT127WT and each of strains M. buxea KCTC 52429T, Massilia armeniaca ZMN-3T, Massilia plicata DSM 17505T and Massilia namucuonensis CGMCC 1.11014T were less than 83.1% and 26.6%, respectively. The reconstructed phylogenomic tree based on concatenated 92 core genes showed that strain F T127WT clusters closely with M. namucuonT T ensis CGMCC 1.11014 . The respiratory quinone of strain FT127W was Q-8. The major fatty acids were C16:1 ω7c, C16:0 and C12:0. The polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol and one unidentified phospholipid. Combining above all characteristics, strain F T127WT should represent a novel species within genus Massilia, for which the name Massilia aquatica sp. nov. (type strain FT127WT = GDMCC 1.1690T = KACC 21482T) is proposed.
Introduction The genus Massilia was proposed by La Scola et al. in 1998 based on the species Massilia timonae which was isolated from blood of an immunocompromised patient with cerebellar lesions [1], and was emended later by Kämpfer et al. in 2011 [2] and Singh et al. in 2015 [3], respectively. At the time of writing, over 40 novel species within this genus were validly published (https: //lpsn.dsmz.de/genus/ massil ia). The The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA genesequence and draft genome sequence of strain FT127WT are MN865813 andWWCU00000000, respectively. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00284-020-02104-1) contains supplementary material, which is available to authorized users. * Mei‑Ying Xu [email protected] 1
Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, People’s Republic of China
2
State Key Laboratory of Applied Microbiology Southern China, Guangzhou 510070, People’s Republic of China
3
Guangdong Open Laboratory of Applied Microbiology, Guangzhou 510070, People’s Republic of China
species within this genus were isolated from blood, ice, rock surface, soil, air and so on [1, 3–9]. Cells are Gram-strainnegative and rod-shaped. Q-8 is the predominant respiratory quinone and the major cellular fatty acids consist of C 16:0 and C16:1 ω7c. The major polar lipids are
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