Pseudidiomarina piscicola sp. nov., isolated from cultured European seabass, Dicenthrarchus labrax
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ORIGINAL PAPER
Pseudidiomarina piscicola sp. nov., isolated from cultured European seabass, Dicenthrarchus labrax M. Carmen Macián1 · Teresa Lucena1 · David R. Arahal1 · María A. Ruvira1 · Rosa Aznar1 · María J. Pujalte1 Received: 16 September 2020 / Revised: 28 October 2020 / Accepted: 18 November 2020 © The Author(s) 2020
Abstract Strain CECT 9734 T, a Gram-negative, aerobic, chemoorganotrophic bacterium, motile by polar flagella, was isolated from cultured European seabass, Dicenthrarchus labrax, in Spain. It grows from 5 to 42 ºC, 6–9 pH and 1–12% total salinity. Major cellular fatty acids are C 15:0 iso, summed feature 9 ( C17:1 iso w9c/C16:0 10-methyl) and C 17:0 iso. The genome size is 2.5 Mbp and G + C content is 49.5 mol%. Comparative analysis of the 16S rRNA gene sequence shows that the strain is a member of Pseudidiomarina, with highest similarities with Pseudidiomarina halophila (97.0%) and Pseudidiomarina salinarum (96.9%). Phylogenomic tree based on UBCG program shows P. halophila as its closest relative. ANI and in-silico DDH with other Pseudidiomarina spp. are lower than 87 and 20%, respectively, suggesting that strain CECT 9734 T represents a new species, for which we propose the name Pseudidiomarina piscicola sp. nov. and CECT 9734 T (= LUBLD50 7aT = LMG 31044 T) as type strain. Keywords Pseudidiomarina · Idiomarina · Idiomarinaceae · Dicenthrarchus labrax · Taxogenomics
Introduction Pseudidiomarina is a genus belonging to the family Idiomarinaceae, proposed by Jean et al. 2006. In the following years, several species were described as members of the genus, including the reclassification of two species of Idiomarina. As more species were incorporated, more difficult was their classification, and finally, Taborda et al. (2009) based on the absence of distinguishing phenotypic characteristics, transferred the nine validly named species of the Communicated by Erko Stackebrandt. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene and the whole genome sequences of Pseudidiomarina piscicola CECT 9734 T are MN809182 and CACRZB010000001CACRZB010000016, respectively. Supplementary Information The online version contains supplementary material available at https://doi.org/10.1007/s0020 3-020-02131-3. * María J. Pujalte [email protected] 1
genus Pseudidiomarina to the genus Idiomarina. Recently, Liu et al. (2018) performed a wide genome-based study of the family Idiomarinaceae, including the genus Aliidiomarina (Huang et al. 2012), and proposed the genus Pseudidiomarina to be restored, establishing a taxonomic reclassification of many species in the family based on genomic analysis. As currently defined, Pseudidiomarina contains sixteen validly named species as stated in https://lpsn.dsmz. de/genus/pseudidiomarina (Parte 2018). One of them, P. maritima, has been recently revealed as a later heterotypic synonym of P. tainanensis (Liu et al. 2020). Pseudidiomarina species comprise Gram-negative rods, strictly aerobic and chemoorganotrophic, mesophilic and halophilic wh
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