Bioluminescence Methods and Protocols
Through the study and application of bioluminescence, scientists have painstakingly harnessed a powerful tool that enables us to seek a deeper understanding of the complex mechanisms underpinning so many vital biologic systems. In this fully revised and u
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IN
M O L E C U L A R B I O L O G Y TM
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
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Bioluminescence Methods and Protocols Second Edition
Edited by
Preston B. Rich and
Christelle Douillet University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
Editors Preston B. Rich Department of Surgery University of North Carolina Division of Trauma & Critical Care 4008 Burnett-Womack Bldg. Chapel Hill, NC 27599-7228 USA [email protected]
Christelle Douillet Department of Surgery University of North Carolina Division of Trauma & Critical Care 90 Manning Drive 6119A Thurston-Bowles Bldg. CB# 7161 Chapel Hill, NC 27599-7161 USA [email protected]
Series Editor John M. Walker University of Hertfordshire Hatfield, Herts UK
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-60327-320-6 e-ISBN 978-1-60327-321-3 DOI 10.1007/978-1-60327-321-3 Springer Dordrecht Heidelberg London New York Library of Congress Control Number: 2009931798 # Humana Press, a part of Springer ScienceþBusiness Media, LLC 1998, 2009 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer ScienceþBusiness Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. Printed on acid-free paper Springer is part of Springer ScienceþBusiness Media (www.springer.com)
Preface Part I I was raised in a redbrick Baltimore row house where summer was marked by the timehonored ritual of firefly-chasing – a backyard tradition that has endured the generations. Amid the excitement, my father often told the story of how, when he was a child, researchers at the Johns Hopkins University had appealed for the systematic capture of live fireflies en masse. Science had engaged the Baltimore youth in an entrepreneurial quest to jar as many lightning bugs as the dwindling light of dusk would permit. The very next morning, each 100-count glass jar of glowing crawling insects could be exchanged at the University for exactly one crisp dollar bill. Unrecognized at the time by my father, his joyous endeavors had contributed in a profound way to the advanced molecular biological techniques that serve as the basis for this textbook. In 1947, William McElroy used extracts from those very fireflies to define the fundamental reaction underlying the mystical phenomenon of luminescence, and published ‘‘The energy source for biolu
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