Cell Cycle Synchronization Methods and Protocols
This second edition volume provides detailed protocols on the theoretical background of cell cycle synchronization procedures and instructions on how to implement these techniques. The chapters in Cell Cycle Synchronization: Methods and Protocols, Second
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Gaspar Banfalvi Editor
Cell Cycle Synchronization Methods and Protocols Second Edition
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Cell Cycle Synchronization Methods and Protocols Second Edition
Edited by
Gaspar Banfalvi Department of Biotechnology and Microbiology, University of Debrecen, Debrecen, Hungary
Editor Gaspar Banfalvi Department of Biotechnology and Microbiology University of Debrecen Debrecen, Hungary
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6602-8 ISBN 978-1-4939-6603-5 (eBook) DOI 10.1007/978-1-4939-6603-5 Library of Congress Control Number: 2016955331 © Springer Science+Business Media New York 2011, 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A
Preface What Kind of Cells Can Be Synchronized? To study how cells progress through the cell cycle, cell cultures have to be brought to the same phase. The unique feature of this book is exactly this: to prepare synchronized cells representing different stages of the cell cycle. The book also shows the latest techniques for the enhanced study of regulatory mechanisms to understand cell cycle events. The synchronization methods presented in the book are based principally on two major strategies. The “arrest-and-release” approach involves different chemical treatments to block cells at certain stages of the cell cycle. The physical strategy contains physical methods to collect cells belonging to subpopulations of the cell cycle. The collection of synchronized cells from asynchronous bacterial, plant, protozoan, yeast, fish, and mammalian cell cultures consisti
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