Complete mitochondrial genome of Atractomorpha sagittaris (Orthoptera: Pyrgomorphidae) and its phylogenetic analysis for

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ORIGINAL ARTICLE

Complete mitochondrial genome of Atractomorpha sagittaris (Orthoptera: Pyrgomorphidae) and its phylogenetic analysis for Acrididea Ran Li 1 & Xiaohan Shu 1 & Wei-an Deng 2 & Ling Meng 1 & Baoping Li 1 Received: 28 July 2019 / Accepted: 19 December 2019 # Institute of Zoology, Slovak Academy of Sciences 2020

Abstract We sequenced the complete mitogenome of Atractomorpha sagittaris (Orthoptera: Pyrgomorphidae) and compared with other Acrididea species. The circular genome was 15,763 bp in size and contained the entire set of 37 genes usually present in orthopteran mitogenomes. The AT-skew of this mitogenome was slightly positive (0.17) and the nucleotide composition biased toward A + T nucleotides (73.38%). All of the 13 PCGs initiated with canonical start codon (ATN), except for COI initiating with ACT. In addition, analyses of pairwise genetic distances between individual PCGs in Acrididea species showed that ND1 was the least conserved gene, while COI the most conserved. Furthermore, we reconstructed phylogenetic trees among species from six superfamiles in Acrididea using Bayesian Interference and Maximum Likelihood methods. Our phylogenetic analyses offered support of monophyly of the superfamilies, and reconfirmed some of species groups including A. sagittaris in Pyrgomorphidae. Keywords Atractomorpha sagittaris . Pyrgomorphidae . Orthoptera . Mitogenome . Phylogeny

Introduction Mitochondrial genome (mitogenome) of insects is usually circular molecule spanning 14–20 kb in size (Wolstenholme 1992; Boore 1999). It generally contains 37 genes, including 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), two ribosomal RNA genes (rRNAs), and a typical control region (A + T-rich region), which contains some initiation sites for transcription and replication of the genome (Zhang and Hewitt 1997; Li et al. 2018). Mitogenomes exhibit several properties, such as small genome size, rapid evolutionary rate, low recombination and maternal inheritance, thus being increasingly employed as valuable molecular markers

Electronic supplementary material The online version of this article (https://doi.org/10.2478/s11756-019-00402-z) contains supplementary material, which is available to authorized users. * Baoping Li [email protected] 1

College of Plant Protection, Nanjing Agricultural University, Nanjing, Jiangsu 210095, People’s Republic of China

2

College of Chemistry and Bioengineering, Hechi University, Yizhou, Guangxi 546300, People’s Republic of China

in the study of species identification, population genetics, comparative and evolutionary genomics, and phylogeny (Galtier et al. 2009; Avise 2009; Cameron 2014; Dai et al. 2018). They are increasingly employed to explore the evolution and phylogenetic relationships in diverse insect taxa (Caterino et al. 2000; McDonagh et al. 2016). The order Orthoptera is highly diverse, including grasshoppers, crickets and katydids, with 28,200 extant species in the world (Cigliano et al. 2018). Among these species, only a tiny number (about 180) have been