Geodermatophilus siccatus sp. nov., isolated from arid sand of the Saharan desert in Chad
- PDF / 270,305 Bytes
- 8 Pages / 547.087 x 737.008 pts Page_size
- 68 Downloads / 176 Views
ORIGINAL PAPER
Geodermatophilus siccatus sp. nov., isolated from arid sand of the Saharan desert in Chad Maria del Carmen Montero-Calasanz • Markus Go¨ker • Manfred Rohde • Peter Schumann • Gabriele Po¨tter • Cathrin Spro¨er • Anna A. Gorbushina Hans-Peter Klenk
•
Received: 13 August 2012 / Accepted: 3 October 2012 / Published online: 18 October 2012 Ó Springer Science+Business Media Dordrecht 2012
Abstract A novel Gram-positive, aerobic, actinobacterial strain, CF6/1T, was isolated in 2007 during environmental screening of arid desert soil in the Sahara near to Ourba, Chad. The isolate was found to grow best in a temperature range of 20–37 °C and at pH 6.0–8.5 and showed no NaCl tolerance, forming black-coloured and nearly circular colonies on GYM agar. Chemotaxonomic and molecular characteristics determined for the isolate match those previously described for members of the genus Geodermatophilus. The DNA G ? C content of the novel strain was determined to be 74.9 mol %. The peptidoglycan was found to contain meso-diaminopimelic acid as the
Electronic supplementary material The online version of this article (doi:10.1007/s10482-012-9824-x) contains supplementary material, which is available to authorized users. M. del Carmen Montero-Calasanz M. Go¨ker P. Schumann G. Po¨tter C. Spro¨er H.-P. Klenk (&) DSMZ – German Collection of Microorganisms and Cell Cultures, Leibniz Institute, Inhoffenstraße 7B, 38124 Braunschweig, Germany e-mail: [email protected] M. del Carmen Montero-Calasanz IFAPA – Instituto de Investigacio´n y Formacio´n Agraria y Pesquera, Centro Las Torres-Tomejil, Ctra. SevillaCazalla de la Sierra, Km 12.2, C.P. 41200 Alcala´ del Rı´o, Sevilla, Spain
diagnostic diamino acid. The main phospholipids were determined to be phosphatidylethanolamine, phosphatidylinositol, phosphatidylcholine, diphosphatidylglycerol and traces of phosphatidylglycerol; MK9(H4) was identified as the dominant menaquinone and galactose as the diagnostic sugar. The major cellular fatty acids were found to be the branched-chain saturated acids iso-C16:0 and iso-C15:0, as well as C17:1x8c. The 16S rRNA gene sequence shows 97.5–97.9 % sequence identity with the four validly named or at least effectively published members of the genus: Geodermatophilus obscurus (97.5 %), Geodermatophilus arenarius (97.7 %), Geodermatophilus ruber (97.9 %) and Geodermatophilus nigrescens (97.9 %). Based on the results from this polyphasic taxonomic analysis and DNA–DNA hybridizations with all type strains of the genus, we propose that A. A. Gorbushina (&) BAM – Federal Institute for Material Research and Testing, 12205 Berlin, Germany e-mail: [email protected] A. A. Gorbushina Department of Biology, Chemistry and Pharmacy, Free University of Berlin, 12249 Berlin, Germany A. A. Gorbushina Department of Earth Sciences, Free University of Berlin, 12249 Berlin, Germany
M. Rohde HZI – Helmholtz Centre for Infection Research, Inhoffenstraße 7, 38124 Braunschweig, Germany
123
450
Antonie van Leeuwenhoek (2013) 103:449–456
strain CF6/1T rep
Data Loading...
