In vitro culture and differentiation of osteoblasts from human umbilical cord blood
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In vitro culture and differentiation of osteoblasts from human umbilical cord blood Tran Cong Toai Æ Huynh Duy Thao Æ Nguyen Phuong Thao Æ Ciro Gargiulo Æ Phan Kim Ngoc Æ Pham Hung Van Æ D. Michael Strong
Received: 29 March 2009 / Accepted: 14 June 2009 / Published online: 30 June 2009 Ó Springer Science+Business Media B.V. 2009
Abstract It is well accepted that human umbilical cord blood (UCB) is a source of mesenchymal stem cells (MSCs) which are able to differentiate into different cell phenotypes such as osteoblasts, chondrocytes, adipocytes, myocytes, cardiomyocytes and neurons. The aim of this study was to isolate MSCs from human UCB to determine their osteogenic potential by using different kinds of osteogenic medium. Eventually, only those MSCs cultured in osteogenic media enriched with vitamin D2 and FGF9, were positive for osteocalcin by RT-PCR. All these cells were positive for alizarin red, alkaline phosphatase and Von Kossa. The results obtained from RT-PCR have confirmed that osteogenesis is T. C. Toai H. D. Thao N. P. Thao C. Gargiulo Department of Histo-pathology, Embryology, Genetics and Biotechnology for Tissue Transplants, Pham Ngoc Thach Medical University, Ho Chi Minh City, Vietnam P. K. Ngoc University of Natural Science, Ho Chi Minh City, Vietnam P. H. Van The Central Lab of the University of Medicine and Pharmacy, Ho Chi Minh City, Vietnam D. M. Strong (&) Department of Orthopaedics and Sports Medicine, University of Washington School of Medicine, Seattle, WA 98195, USA e-mail: [email protected]
complete by expression of the osteocalcin marker. In conclusion, vitamin D2, at least in vitro, may replace vitamin D3 as an osteogenic stimulator factor for MSC differentiation. Keywords Mesenchymal stem cells (MSCs) Human umbilical cord (UCB) Bone marrow (BM) Major histocompatibility complex (MHC)
Introduction Multipotent mesenchymal stem cells Mesenchymal stem cells (MSCs) are a particular type of cell of embryonic mesodermal origin with a strong adherence ability and capable of differentiating into cells of different lineage tissues such as bone, cartilage, adipose tissues (Lee et al. 2004; Park et al. 2006; Bieback et al. 2004; Boissy et al. 2000; Chamberlain et al. 2007), and neural cells including astrocytes, neurons, hepatocystic and dermal tissue (Tse and Laughlin 2005; Chao et al. 2004; Koc and Lazarus 2001; Xu et al. 2004; Minguell et al. 2001; Song and Tuan 2004; Goodwin et al. 2001; Rosada et al. 2003; Kogler and Wernet 2006; Riordan et al. 2007; Kim et al. 2004; Jang et al. 2006; Kang et al. 2006; Van de Ven et al. 2007). Human MSCs have been isolated from various sources, such as adipose tissues, bone marrow (BM), umbilical cord blood (UCB), amniotic fluid, amniotic placenta, scalp tissue, amniotic membrane,
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and synovial tissue (Chao et al. 2004; Koc and Lazarus 2001; Xu et al. 2004; Minguell et al. 2001; Song and Tuan 2004; Goodwin et al. 2001; Rosada et al. 2003; Kogler and Wernet 2006; Riordan et al. 2007; Koblas et a
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