LED Exposure Modulates the Biosynthesis of Silver Nanoparticles from Root Tuber Extract of Chlorophytum borivilianum and
- PDF / 3,532,663 Bytes
- 14 Pages / 595.276 x 790.866 pts Page_size
- 85 Downloads / 212 Views
(0123456789().,-volV)(0123456789(). ,- volV)
ORIGINAL PAPER
LED Exposure Modulates the Biosynthesis of Silver Nanoparticles from Root Tuber Extract of Chlorophytum borivilianum and their Phytotoxicty S. Dutta Gupta1 • P. Kohli1 Received: 27 June 2020 / Accepted: 17 September 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract The present study reports an efficient phyto-mediated and photo-catalyzed synthesis of silver nanoparticles (AgNPs) from root tuber extract of Chlorophytum borivilianum and demonstrates its phytotoxicity during seedling growth of Peltophorum pterocarpum. A time course analysis of the appearance of SPR peak during incubation of reaction mixture under the exposure of various light treatments suggested the influence of light spectral quality on the biosynthesis of AgNPs. Irradiation of reaction mixture with blue LEDs (BL) induced the rapid synthesis of spherical particles smaller in size compared to other treatments. The reaction mixture optimized for the synthesis of AgNPs remains same for all the exposure conditions. FTIR spectra indicated the presence of steroidal saponins and their influence in bioreduction and capping of biosynthesized AgNPs. A bioreduction mechanism insensitive to the variations in spectral quality was proposed suggesting borivilianoside H, a steroidal saponin, as the bioreductant. The toxic impact of AgNPs was more pronounced with the particles synthesized under BL exposure particularly at rooting stage. The findings suggest the influence of LED lighting not only on the biosynthesis of AgNPs but also on its phytotoxicity. Keywords Spectral quality Photobioreduction Borivilianoside-H Phytotoxicity Surface plasmon resonance peak
Introduction Silver nanoparticles (AgNPs) with their unique physical, optical, electronic, catalytic and biological properties have been exploited in various fields with potential applications, such as biosensors, antibacterial agents, anti-cancer therapeutics, water purification, food preservation, diagnostics and optoelectronics [1, 2]. With increasing quest on the toxic as well as phytostimulatory impact of AgNPs to plant ecosystem [3–5], bio-inspired synthesis of AgNPs has received special attention over both the top-down and bottom-up approaches of physical and chemical methods [2]. Compared to physical and chemical methods, Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10876-020-01899-0) contains supplementary material, which is available to authorized users. & S. Dutta Gupta [email protected] 1
Agricultural and Food Engineering Department, Indian Institute of Technology, Kharagpur 721302, India
biosynthesis of AgNPs utilizing extract of different plant parts from a variety of plant species has recognised as a simple and cost effective alternative owing to its nonhazardous and eco-friendly nature. Plant based synthesis of metal nanoparticles is preferred over the microbial systems as it eliminates the process of establishment of microbial culture a
Data Loading...
