Liposomes Methods and Protocols
This second edition volume expands on the previous edition by discussing classic techniques, as well as new protocols that focus on the preparation of liposomes, lipid characterization, particle size and charge analysis, drug encapsulation, surface modifi
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Gerard G.M. D’Souza Editor
Liposomes Methods and Protocols Second Edition
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Liposomes Methods and Protocols Second Edition
Edited by
Gerard G.M. D’Souza School of Pharmacy – Boston, MCPHS University, Boston, MA, USA
Editor Gerard G.M. D’Souza School of Pharmacy – Boston MCPHS University Boston, MA, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6589-2 ISBN 978-1-4939-6591-5 (eBook) DOI 10.1007/978-1-4939-6591-5 Library of Congress Control Number: 2016955241 © Springer Science+Business Media New York 2010, 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Whether it is as convenient platforms for the study of membrane dynamics and membrane proteins or as potential carrier systems for drug delivery, liposomes are constantly being utilized in innovative ways as new analytical and synthetic technologies become available. When I was invited to edit this second edition of MiMB Liposomes Methods and Protocols, my initial thoughts went immediately to a focus on new liposome applications and protocols. However, the more I thought about my vision for this book and the audience I wanted to reach, the more I felt that a focus on basic protocols was warranted. As with any system that has been around as long as liposomes have, early knowledge often tends to be lost from everyday use as trends change and new investigators with diverse backgrounds enter the field. Basic protocols and concepts often get abbreviated or even omitted from the latest publications. Consequently investigators
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