Mammalian Cell Viability Methods and Protocols
Whether the question is one of basic cell survival, or whether it is being used to correlate cell number to some other factor such as matrix synthesis, an estimate of cell viability is universally required. In Mammalian Cell Viability: Methods and P
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Molecular Biology™
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
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Mammalian Cell Viability Methods and Protocols
Edited by
Martin J. Stoddart AO Research Institute Davos, Davos Platz, Switzerland
Editor Martin J. Stoddart, Ph.D. AO Research Institute Davos Davos Platz, Switzerland [email protected]
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-61779-107-9 e-ISBN 978-1-61779-108-6 DOI 10.1007/978-1-61779-108-6 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2011925550 © Springer Science+Business Media, LLC 2011 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. While the advice and information in this book are believed to be true and accurate at the date of going to press, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with respect to the material contained herein. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
Preface Viability of cells is one of the most fundamental measurements made during studies in cell biology. Whether the question is one of basic cell survival, or whether it is being used to correlate cell number to some other factors such as matrix synthesis, an estimate of cell viability is universally required. The specific method used will greatly influence the interpretation of the data. While many viability methods have been used for decades, there have been recent developments which offer increased sensitivity, throughput, and specificity. The particular type of cell death, apoptotic or necrotic, is becoming increasingly important. This requires multiplexing of methods, or methods that are able to distinguish between the different cell states. This book aims to bring together a wide array of methods in order to assist the reader to determine which is most suitable for them. Certain methods will provide information about the population as a whole, while others determine viability on single cell level. In some cases, it may also be important to realize that the method, while producing an answer, is not suitable for t
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