The CRAC Channel Methods and Protocols
This volume discusses the cellular and molecular techniques used to study and characterize the different components of the CRAC channel signaling pathway. The chapters in this book cover topics such as fluorescence-based measurements of the CRAC channel a
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Aubin Penna Bruno Constantin Editors
The CRAC Channel Methods and Protocols
Methods
in
M o l e c u l a r B i o lo g y
Series Editor: John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
The CRAC Channel Methods and Protocols
Edited by
Aubin Penna IRSET Inserm UMR 1085/ UR1, Rennes, France
Bruno Constantin CNRS ERL 7003, Laboratoire STIM, Poitiers, France
Editors Aubin Penna IRSET Inserm UMR 1085/ UR1 Rennes, France
Bruno Constantin CNRS ERL 7003 Laboratoire STIM Poitiers, France
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-8702-3 ISBN 978-1-4939-8704-7 (eBook) https://doi.org/10.1007/978-1-4939-8704-7 Library of Congress Control Number: 2018951920 © Springer Science+Business Media, LLC, part of Springer Nature 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors, and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC part of Sprinter Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Cytosolic and endoplasmic reticulum Ca2+ levels are closely regulated in all cells by transport mechanisms including pumps and store-operated Ca2+ channels that allow Ca2+ influx across the plasma membrane when the ER luminal Ca2+ store is depleted. In particular, an inwardly rectifying, highly Ca2+-selective store-operated current that was identified in the late 1980s by patch-clamp electrophysiology plays a major role in T lymphocytes by regulating the triggering, intensity, and duration of Ca2+ signaling leading to downstream changes in gene expression and cell proliferation. Named the Ca2+ release-activated Ca2+ (CRAC) current, the molecular identity of the channel sustaining this influx and the exac
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