Zygotic Genome Activation Methods and Protocols
This volume provides an overview of zygotic genome activation (ZGA) and the use of recent tools to elucidate the events that occur during ZGA. The chapters in this book cover topics such as: clearance of maternal RNAs, detection of miRNA in mammalian oocy
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Kiho Lee Editor
Zygotic Genome Activation Methods and Protocols
Methods
in
Molecular Biology
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Zygotic Genome Activation Methods and Protocols
Edited by
Kiho Lee Department of Animal and Poultry Sciences, Virginia Tech, Blacksburg, VA, USA
Editor Kiho Lee Department of Animal and Poultry Sciences Virginia Tech Blacksburg, VA, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6986-9 ISBN 978-1-4939-6988-3 (eBook) DOI 10.1007/978-1-4939-6988-3 Library of Congress Control Number: 2017937534 © Springer Science+Business Media LLC 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Proper embryogenesis requires well-orchestrated events. After fertilization, initially maternal factors stored in the egg lead the development and the zygotic genome is dormant. Then, zygotic genome controls the development by initiating its own transcription. Successful transition into this event, zygotic genome activation (ZGA), is critical for embryo survival. Previous studies have demonstrated that dramatic degradation of maternal mRNA occurs and activation of specific zygotic genes is involved during ZGA. However, specific pathways and factors involved in the process have not been fully elucidated. One of the main obstacles to investigating the process is limited tools available for molecular analyses of the event. Specifically, due to the limited amount of samples (DNA, RNA, and protein) available from e
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