Lentzea indica sp. nov., a novel actinobacteria isolated from Indian Himalayan-soil
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ORIGINAL PAPER
Lentzea indica sp. nov., a novel actinobacteria isolated from Indian Himalayan-soil Pulak Kumar Maiti . Sukhendu Mandal
Received: 20 April 2020 / Accepted: 11 July 2020 Springer Nature Switzerland AG 2020
Abstract A novel actinobacterium, strain PSKA42T was isolated from a soil sample of Kashmir Himalaya, India (latitude: 34-010 N; longitude: 74-470 E; altitude: 5328 ft). The morphological, biochemical and molecular characteristics predicted that PSKA42T belongs to the genera Lentzea. Using the Ezbiocloud server it has also been manifested that the 16S rRNA gene sequence of PSKA42 was 98.4% similar with Lentzea guizhouensis DHSC013T, Lentzea albidocapillata subsp. violacea IMSNU50388T; 98.26% similar with Lentzea aerocolonigenes NRRLB3298T, Lentzea albidocapillata subsp. albidocapillata NRRLB-24057T and 98.19% similar with Lentzea californiensis DSM43393T. Whole-genome average nucleotide identity values between PSKA42T and L. guizhouensis DHSC013T, L. albidocapillata subsp. violaera IMSNU50388T, L. aerocolonigenes NRRLB-
The GenBank accession number for the 16S rRNA gene sequence of strain PSKA42T is MH299826. The Whole Genome sequence has been deposited at GenBank under the Accession Number VSRL00000000.
3298T, L. albidocapillata subsp. albidocapillata NRRLB-24057T, L. californiensis DSM43393T, L. flaviverucosa As40578T were 84.94%, 85.8%, 87.15%, 87.71%, 79.29, and 87.22%, respectively, suggesting that PSKA42T represented a new species. In-silco genome-to-genome distances analysis and MLSA with selected housekeeping genes also indicated that the isolate should be assigned to a new species under the genus Lentzea. Analysis of the whole-cell hydrolysate found mannose and ribose as major sugar while cell wall amino acid was identified as meso-diaminopimelic acid. Major fatty acids ([ 5%) were observed as 15:0 iso (23.81%), and 16:0 iso (26.11%). PSKA42 contains polyketide synthase type-I genes but unable to show antimicrobial activity in laboratory conditions. The average GC content in its genome is 68.3%. Further, based on physicochemical, chemotaxonomic markers and molecular analysis, PSKA42T can be assigned as novel species of Lentzea, we proposed Lentzea indica sp nov. The type strain is PSKA42T (JCM 33729T, MTCC 12936T, MCC 4127T).
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10482-020-01449-8) contains supplementary material, which is available to authorized users.
Keywords Actinomycetes ANI Himalayan-soil Lentzea MLSA
P. K. Maiti S. Mandal (&) Laboratory of Molecular Bacteriology, Department of Microbiology, University of Calcutta, 35, Ballygunge Circular Road, Kolkata 700019, India e-mail: [email protected]
Abbreviations BA Bennett’s agar ISP International streptomyces project GGDC Genome-to-genome distance calculator
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Antonie van Leeuwenhoek
MLSA ANI NRPS PKS
Multilocus sequence analysis Average nucleotide identity Non ribosomal polypeptide synthetases Polyketide
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