Arthrobacter sedimenti sp. nov., isolated from river sediment in Yuantouzhu park, China
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ORIGINAL PAPER
Arthrobacter sedimenti sp. nov., isolated from river sediment in Yuantouzhu park, China Pei Lin1 · Zheng‑Fei Yan2 · Chang‑Tian Li3 Received: 9 May 2020 / Revised: 19 June 2020 / Accepted: 24 June 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract A Gram-stain positive, motile, aerobic and rod-shaped strain (MIC A 30T) was isolated from river sediment in Yuantouzhu park, Wuxi City, China. Growth occurred at 20–40 °C, at pH 6.0–9.0 and at 0–5.0% NaCl. Strain MIC A 30T was moderately T T related to Arthrobacter liuii CGMCC 1.12778 (97.9%), Arthrobacter pokkalii (97.9%) and Arthrobacter globiformis NBRC 12137T (96.7%) by 16S rRNA analysis. The DNA–DNA relatedness values between strain MIC A30T and these reference strains were below 30%. The DNA G+C content was 63.1 mol%. Average nucleotide identity (ANI) and genome-to-genome distance (GGD) values between strain MIC A30T and A. liuii CGMCC 1.12778T were 60.34% and 29.39%, respectively. Quinone was identified as MK-9(H2). Major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. Major fatty acids were iso-C15:0, anteiso-C15:0 and anteiso-C17:0. Whole-cell sugars were galactose, mannose and rhamnose. The cell wall peptidoglycan contained A4α peptidoglycan type with lysine as the diagnostic diamino acid. Based on several taxonomic results, strain MIC A30T is identified as a novel species in genus Arthrobacter, whose name is proposed as Arthrobacter sedimenti sp. nov. The type strain is MIC A30T (= KACC 19599T = CGMCC 1.13474T). Keywords Arthrobacter sediment · Taxonomy · River sediment · Phenotypic characteristics
Introduction The genus Arthrobacter was established (Conn and Dimmick 1947), and emended (Koch et al. 1995) as member of the family Micrococcaceae, which has been subjected to several taxonomic revisions during the last few years (Busse 2016). Communicated by Erko Stackebrandt. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-020-01968-y) contains supplementary material, which is available to authorized users. * Zheng‑Fei Yan [email protected] * Chang‑Tian Li [email protected] 1
School of Pharmaceutical Sciences, Jiangnan University, 1800 Lihu Ave, Wuxi 21422, China
2
State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Ave, Wuxi 21422, China
3
Engineering Research Center of Edible and Medicinal Fungi, Ministry of Education, Jilin Agricultural University, Changchun 130118, China
Currently, genus Arthrobacter is one of the most divergent heterotrophic bacteria in actinomycetes, which contains more than 120 validly published species (https://lpsn.dsmz. de/genus/arthrobacter). Most members have been isolated from various environments (Krishnan et al. 2016; Yu et al. 2015), such as plants, soils, specimens and freshwater. Due to significant difference in 16S rRNA gene sequence and cell components such as peptidoglycans, quinones, polar lipids, selected species were reclassified into seve
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