Arthrobacter sulfonylureivorans sp. nov., isolated from a sulfonylurea herbicides degrading consortium enriched with bir
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ORIGINAL PAPER
Arthrobacter sulfonylureivorans sp. nov., isolated from a sulfonylurea herbicides degrading consortium enriched with birch forest soil Xiaoyan Han1,2 · Qi Zhang3 · Qingyun Ma1 · Delong Kong1 · Yiqing Zhou1 · Xu Jiang1,5 · Wei Zhang3 · Zhiyong Ruan1,4 Received: 10 March 2020 / Revised: 13 October 2020 / Accepted: 16 October 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract A gram-stain positive, aerobic, motile, rod-shaped bacterium, designated strain LAM7117T, was isolated from a sulfonylurea herbicides degrading consortium enriched with birch forest soil. The optimal temperature and pH for the growth of strain LAM7117T were 35 °C and 7.5, respectively. Strain LAM7117T could grow in the presence of NaCl with concentration up to 9% (w/v). Strain LAM7117T formed a distinct phylogenetic subclade within the genus Arthrobacter in the phylogenetic trees built with 16S rRNA gene sequences and shared the highest similarity with A. crystallopoietes JCM 2522T (97.7%). The values of digital DNA–DNA relatedness and Avery Nucleotide Identity based on the genome sequences between L AM7117T T and A. crystallopoietes JCM 2522 were 21.4 and 77.4%, respectively. The genomic DNA G + C content was 65.9 mol%. The major cellular fatty acids were anteiso-C15:0, iso-C16:0 and anteiso-C17:0. The cell wall peptidoglycan contained the amino acids as glycine, lysine, alanine and glutamic acid. The major polar lipids present in strain L AM7117T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidyl inositol, two unidentified glycolipids and one unidentified lipid. The predominant menaquinones of strain L AM7117T were MK-8 and MK-9. Based on the phenotypic characteristics, chemotaxonomic data and genotypic analyses, strain L AM7117T should be classified as a novel species of genus Arthrobacter, for which the name Arthrobacter sulfonylureivorans sp. nov. is proposed. The type strain is LAM7117T (= JCM 32824T = CGMCC 1.16681T). Keywords Arthrobacter · 16S rRNA gene · Polyphasic taxonomy · Forest soil Abbreviations JCM Japan collection of microorganisms ANI Average nucleotide identity
Communicated by Erko Stackebrandt. Xiaoyan Han, Qi Zhang and Qingyun Ma contributed equally to this work. Electronic Supplementary Material The online version of this article (https://doi.org/10.1007/s00203-020-02097-2) contains supplementary material, which is available to authorized users. * Wei Zhang [email protected] * Zhiyong Ruan [email protected] Extended author information available on the last page of the article
Introduction The genus Arthrobacter belongs to the family Micrococcaceae and was proposed by Conn and Dimmick (1947). Strains of the genus Arthrobacter have been isolated from various environmental sources, such as soil, clinical environments, marine sediment, cheese, mural paintings, water, sewage, the air, cyanobacterial mat and filtration substrate (Skyring and Quadling 1970; Pindi et al. 2010; Li et al. 2004). At the time of writing, the genus Arthrobacter comprises 100
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