Caenimonas sedimenti sp. nov., Isolated from Sediment of the Wastewater Outlet of an Agricultural Chemical Plant
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REVIEW ARTICLE
Caenimonas sedimenti sp. nov., Isolated from Sediment of the Wastewater Outlet of an Agricultural Chemical Plant Jianyi Xu1 · Mengyao Sheng1 · Zhou Yang1 · Jiguo Qiu1 · Jun Zhang2 · Lei Zhang3 · Jian He1 Received: 14 March 2020 / Accepted: 22 July 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract A novel bacterial strain, designated HX-9-20T, was isolated from the sediment collected from the wastewater outlet of an agricultural chemical plant in Maanshan city, Anhui province, PR China. Cells of strain HX-9-20T were Gram-stainingnegative, rod-shaped, translucent, non-motile, and strictly aerobic. Growth was observed between 15 and 35 °C (optimum 30 °C), at pH 6.0–8.0 (optimum pH 7.0), and in the presence of 0–0.4% (w/v) NaCl (optimum 0.2%). The predominant cellular fatty acids were summed feature 3 ( C16:1 ω7c and/or C 16:1 ω6c), C 16:0 and summed feature 8 ( C18:1 ω6c and/or C 18:1 ω7c). The major quinone was ubiquinone Q-8. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, and an aminophospholipid. Strain HX-9-20T contains 2-hydroxyputrescine and putrescine as the major polyamine. A phylogenetic analysis based on 16S rRNA gene sequences revealed that strain HX-9-20T was affiliated with the genus Caenimonas, exhibiting the highest sequence similarities with Caenimonas koreensis EMB320T (97.3% similarity) and Ramlibacter humi 18×22-1T (97.0%), and less than 97.0% similarity with other type strains. The average nucleotide identity (ANI) and digital DNA–DNA hybridization values (dDDH) between HX-9-20T and C. koreensis EMB320T were 76.9% and 23.5% respectively. The ANI and dDDH between HX-9-20T and R. humi 18×22-1T were 80.3% and 23.6%, respectively. The G + C content of the genomic DNA was 67.5 mol%. On the basis of the polyphasic taxonomic data, strain HX-9-20T represents a novel species of the genus Caenimonas, for which the name Caenimonas sedimenti sp. nov. is proposed. The type strain is HX-9-20T (=KCTC 72473T=CCTCC AB 2019266T).
Introduction The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences and the whole genome of strains HX-9-20T are MN317366 and VOBQ00000000, respectively. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00284-020-02145-6) contains supplementary material, which is available to authorized users. * Jun Zhang [email protected] * Jian He [email protected] 1
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Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China
The genus Caenimonas belongs to the family Comamonadaceae, class Betaproteobacteria [1]. As of May 2020, the genus Caenimonas comprises two species with validly published names, C. koreensis EMB320T [2] and C. terrae SGM1-15T [3] (https://www.bacter io.net/caenimonas .html) [4]. The type strain C. koreensis EMB320T was isolated from activated sludge treating phosphate-containing wastewater
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