Aureimonas psammosilene sp. nov., isolated from the roots of Psammosilene tunicoides
- PDF / 1,046,294 Bytes
- 6 Pages / 595.276 x 790.866 pts Page_size
- 37 Downloads / 171 Views
ORIGINAL PAPER
Aureimonas psammosilene sp. nov., isolated from the roots of Psammosilene tunicoides Mei‑Juan Huang1 · Wu‑Lue Huang1 · Manik Prabhu Narsing Rao2 · Min Xiao2 · Hai‑Quan Huang1 · Wen‑Jun Li2,3 Received: 29 November 2019 / Revised: 15 March 2020 / Accepted: 1 April 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract One motile strain designated, YIM DR1026T was isolated from the roots of Psammosilene tunicoides collected from Gejiu, Yunnan province, China. The cells of strain YIM D R1026T were Gram-negative and short-rod shaped. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain YIM DR1026T was a member of the genus Aureimonas and closely related to Aureimonas rubiginis (96.7%). DNA–DNA relatedness values between strain YIM 1026T and Aureimonas rubiginis BCRC 8 0440T was 38.2 ± 1.5%. The ANI value between YIM D R1026T and other Aureimonas members were below the cut-off level (95–96%) recommended as the average nucleotide identity (ANI) criterion for interspecies identity. Strain YIM D R1026T grew at 4–30 °C (optimum 28 °C), pH 4.0–9.0 (optimum pH 6.0–7.0) and tolerated NaCl (w/v) up to 1% (optimum 0%). Q-10 was sole the respiratory ubiquinone present in YIM D R1026T. Polar lipids of strain YIM D R1026T were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, sulfoquinovosyldiacylglycerol, unidentified aminolipid and unidentified polar lipid. The genomic G + C content was 64.6 mol%. The major fatty acids were C 18:1 ω7c, C16:0 and summed feature 3 ( C16:1ω7c/C16:1ω6c). Based on phenotypic, phylogenetic, chemotaxonomic and genome comparison, strain YIM D R1026T represents a novel species of the genus Aureimonas, for which the name Aureimonas psammosilene sp. nov. is proposed. The type strain is YIM DR1026T (= KCTC 42691T = NBRC 112412T). Keywords Aureimonas psammosilene sp. nov. · Psammosilene tunicoides · Roots · Polyphasic taxonomy Abbreviation ANI Average nucleotide identity Communicated by Erko Stackebrandt. Mei-Juan Huang and Wu-Lue Huang have contributed equally to this work. The 16S rRNA gene and genome sequence of strain YIM DR1026T has been deposited in GenBank under the accession numbers MK278899 and WEZG00000000, respectively. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-020-01872-5) contains supplementary material, which is available to authorized users.
Introduction Rathsack et al. (2011) divided the genus Aurantimonas into two genera, Aurantimonas and Aureimonas based on 16S rRNA gene sequence analyses and the presence or absence of the glycolipid (sulfoquinovosyldiacylglycerol). Members of the genus Aureimonas were characterized as Gram-negative, rod-shaped, motile, and show the presence of sulfoquinovosyldiacylglycerol (Rathsack et al. 2011). At the time of writing, the genus Aureimonas includes 13 species (https:// www.bacter io.net/aureimonas.html) isolated from various environments such as subterranean cave
Data Loading...
