Achromobacter aestuarii sp. nov., Isolated from an Estuary
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Achromobacter aestuarii sp. nov., Isolated from an Estuary Si Chul Kim1 · Sang Ok Chung2 · Hyo Jung Lee1 Received: 5 March 2020 / Accepted: 29 September 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract A novel strain KS-M25T was isolated from estuary water in South Korea. Strain KS-M25T was Gram-staining-negative, strictly aerobic, motile rods-shaped bacterium and showed oxidase- and catalase-positive reactions. Growth of strain KS-M25T was observed at 10–25 °C (optimum, 20 °C), at pH 5.5–9.0 (optimum, pH 7.5), and with 0–6.0% (w/v) NaCl (optimum, 1%). Ubiquinone-8 was identified as the sole isoprenoid quinone and the major fatty acids were C16:0, cyclo– C17:0 and sum in feature 3 (comprising C16:1 ω7c and/or C16:1 ω6c). The G+C content values based on genome sequences was 62.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain KS-M25T formed a clear cluster within the genus Achromobacter. Strain KS-M25T was most closely related to Achromobacter anxifer LMG 26857T, Achromobacter dolens LMG 2 6840T, and Achromobacter xylosoxidans NBRC 1 5126T with 97.8%, 97.8%, and 97.7% 16S rRNA gene sequence similarities, respectively. Based on phenotypic, chemotaxonomic, and phylogenetic analysis, strain KS-M25T represents a novel species of the genes Achromobacter, for which the name Achromobacter aestuarii sp. nov. is proposed. The type strain is KS-M25T (= KACC 21219T = JCM 33329T).
Introduction The genus Achromobacter, belonging to the family Alcaligenaceae of the class Betaproteobacteria, was first proposed by Bergey et al. [1] with A. liquefaciens as the type species. In the Approved Lists of Bacterial Names in 1980 [2], the generic name Achromobacter was excluded because the lack of an identifiable culture of ‘A. liquefaciens’. Yabuuchi and Yano [3] proposed the name Achromobacter with a new type species Achromobacter xylosoxidans. Then, the genus Achromobacter was emended by Yabuuchi et al. [4] and Alcaligenes ruhlandii [5], Alcaligenes piechaudii [6], and Alcaligenes denitrificans [7] were transferred from the genus Alcaligenes to the genus Achromobacter based on a polyphasic taxonomic characterization. At the time of writing, 22 valid species have been reported as the members of Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00284-020-02231-9) contains supplementary material, which is available to authorized users. * Hyo Jung Lee [email protected] 1
Department of Biology, Kunsan National University, 558 Daehak‑ro, Gunsan‑si 54150, Republic of Korea
Tidal Flat Research Center-National Institute of Fisheries Science, Gunsan, Republic of Korea
2
the genus Achromobacter. These species have been isolated from clinical samples and other environmental samples such as soil, deep subseafloor sediment, the root of Aloe vera, rhizosphere of Panax ginseng, and mouse tissue [https:// lpsn.dsmz.de/genus/achromobacter]. In this study, we isolated a novel strain from estuary water and described its
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