Actinoplanes solisilvae sp . nov., Isolated from Birch Forest Soil
- PDF / 774,151 Bytes
- 8 Pages / 595.276 x 790.866 pts Page_size
- 31 Downloads / 161 Views
Actinoplanes solisilvae sp. nov., Isolated from Birch Forest Soil Qingyun Ma1 · Qi Zhang1,2,5 · Xu Jiang1 · Delong Kong1 · Xiaoyan Han1 · Huiying Xue3 · Yiqing Zhou1 · Yuqin Zhang4 · Wei Zhang2 · Zhiyong Ruan1,3 Received: 5 December 2019 / Accepted: 1 September 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract A novel actinomycete, designated strain LAM7112T, was isolated from soil sample collected from a birch forest in Xinjiang Uygur Autonomous Region, China. The new isolate was found to be able to grow at 20–45 °C (optimum: 35 °C), pH 5.0–10.0 (optimum: 7.0) and in the presence of 0–10.0% (optimum: 3.0%) (w/v) NaCl. The isolate formed very scantily irregular sporangia containing motile spores on the substrate mycelium. Phylogenetic analysis of 16S rRNA gene sequences indicated that the new isolate was closely related to members of the family Micromonosporaceae, with highest similarites to Actinoplanes ferrugineus X-14695T (97.4%), Micromonospora zamorensis DSM 45600T (97.3%) and Micromonospora aurantiaca ATCC 27029T (97.3%). In the phylogenetic trees, strain L AM7112T formed a stable phylogenetic subclade within the genus Actinoplanes. The genomic DNA G + C content was 70.0 mol%. The major fatty acids (> 10%) were determined to be iso-C16:0, anteiso-C15:0 and anteiso-C17:0. The predominant menaquinones were identified as MK-9 (H2), MK-9 (H4) and MK-9 ( H6). The major polar lipids were found to be diphosphatidylglycerol, phosphatidylinositol and phosphatidylethanolamine. The diagnostic amino acid of the cell wall peptidoglycan was determined to be meso-diaminopimelic acid. The diagnostic sugars in cell hydrolysates were determined to be glucose and ribose. On the basis of its phenotypic, phylogenetic and chemotaxonomic characteristics, strain L AM7112T (= CGMCC 4.7580T = JCM 32512T) is proposed to represent the type strain of a novel species of the genus Actinoplanes, for which the name Actinoplanes solisilvae is proposed.
Introduction
Qingyun Ma and Qi Zhang have contributed equally to this study and share first authorship. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strainLAM7112T is MF784352. The GenBank accession number for the draft genome sequence ofstrain LAM7112T is RJAC00000000. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00284-020-02192-z) contains supplementary material, which is available to authorized users. * Wei Zhang [email protected] * Zhiyong Ruan [email protected] 1
2
Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China College of Life Science, Xinjiang Normal University, Ürümqi 830046, Xinjiang, China
Tetracycline is a broad-spectrum antibiotic widely used forhuman and animal health [19]. Because antibiotics cannot be absorbed or metabolized entirely by the animal, antibiotics administered are excreted into the environment. Tetracycline compounds and their transformation products can dis
Data Loading...
