Altererythrobacter segetis sp. nov., Isolated from Farmland Soil
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Altererythrobacter segetis sp. nov., Isolated from Farmland Soil Jae‑Chan Lee1,2 · Kyung‑Sook Whang1,2 Received: 3 August 2020 / Accepted: 11 November 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract A Gram-stain-negative bacterium, designated Y J20T, was isolated from rhizosphere soil of a spinach farmland at Shinan T in Korea. Strain Y J20 was found to be aerobic, non-motile rods which can grow at 10–33 °C (optimum, 28 °C), at pH 6.5–8.5 (optimum, pH 6.5–7.5) and in the absence of NaCl. The 16S rRNA gene sequence analysis showed that strain YJ20T belongs to the genus Altererythrobacter with moderate sequence similarities to Altererythrobacter dongtanensis KCTC 22672T (96.8%), Altererythrobacter soli MN-1T (96.6%) and Altererythrobacter xinjiangensis S3-63T (96.5%). The phylogenomic analysis based on the whole-genome sequence demonstrated that strain Y J20T formed a distinct phyletic line T T with Altererythrobacter soli MN-1 and Altererythrobacter salegens XY-R17 showing average nucleotide identity (ANI) values of 79.4 and 77.5%, respectively. The predominant ubiquinone was identified as Q-10, and the major fatty acids were C17:1 ω6c, C18:1 ω7c and C15:0 2-OH. The major polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidycholin, sphingoglycolipid, an unidentified glycolipid, an unidentified phospholipid and an unidentified lipid. The G+C content of the genome was determined to be 66.3 mol%. On the basis of phenotypic, chemotaxonomic properties and phylogenetic and phylogenomic analyses in this study, strain YJ20T is considered to represent a novel species in the genus Altererythrobacter, for which the name Altererythrobacter segetis sp. nov. is proposed. The type strain is YJ20T (= KACC 19554T = NBRC 113199T). Abbreviations R2A agar Reasoner’s 2A agar dDDH Digital DNA–DNA hybridization ANI Average nucleotide identity
Introduction The genus Altererythrobacter was first proposed by Kwon et al. [1] with the description of Altererythrobacter epoxidivorans as the type species of the genus and subsequently emended by Xue et al. [2], Xue et al. [3] and Lee et al. [4]. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00284-020-02294-8) contains supplementary material, which is available to authorized users. * Kyung‑Sook Whang [email protected] 1
Institute of Microbial Ecology and Resources, Mokwon University, 88 Doanbuk‑ro, Seo‑gu, Daejeon 35349, Republic of Korea
Department of Microbiology & Resources, College of Science & Technology, Mokwon University, 88 Doanbuk‑ro, Seo‑gu, Daejeon 35349, Republic of Korea
2
Currently, the genus Altererythrobacter comprises 43 species with validly published names (www.bacter io.net/genus/ altererythrobacter). Members of the genus Altererythrobacter are mainly distributed in marine and soil environments. Ten species have been isolated from different soils, such as rhizosphere, mountain, wetland, desert,
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