Chitinophaga agri sp. nov., a bacterium isolated from soil of reclaimed land
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ORIGINAL PAPER
Chitinophaga agri sp. nov., a bacterium isolated from soil of reclaimed land Shin Ae Lee1 · Jun Heo1 · Tae‑Wan Kim1 · Mee‑Kyung Sang1 · Jaekyeong Song1 · Soon‑Wo Kwon1 · Hang‑Yeon Weon1 Received: 8 May 2020 / Revised: 14 September 2020 / Accepted: 30 September 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract A Gram-negative, aerobic, and long rod-shaped bacterium, designated as H33E-04T, was isolated from the soil of reclaimed land, Republic of Korea. The strain grew at a temperature range of 15–40 °C, pH 5.0–10.0, and 0–2% NaCl (w/v). The phylogenetic analysis based on 16S rRNA gene sequences showed that strain H33E-04T was in the same clade with Chitinophaga pinensis DSM 2 588T, Chitinophaga filiformis IFO 1 5056T, and Chitinophaga ginsengisoli Gsoil 0 52T with 98.4%, 97.9%, and 97.8% sequence similarities, respectively. The de novo genome assembly revealed that the DNA G + C content of the strain was 46.2 mol%. Comparative genome analysis between strain H33E-04T and C. pinensis DSM 2 588 T showed that the average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values were 79.9% and 23.4%, respectively. The major respiratory quinone was menaquinone-7 (MK-7) and the predominant cellular fatty acids were iso-C15:0 (31.7%), C16:1 ω5c (31.2%), and iso-C17:0 3-OH (11.8%), supporting the affiliation of strain H33E-04T with the genus Chitinophaga. Based on phylogenetic, physiological, and chemotaxonomic characteristics, strain H33E-04T represents a novel species of the genus Chitinophaga, for which the name Chitinophaga agri sp. nov. is proposed. The type strain of Chitinophaga agri is H33E-04T (= KACC 21303T = NBRC114512T). Keywords Chitinophaga · Reclaimed land · Novel species · Polyphasic taxonomy
Introduction The genus Chitinophaga was proposed by Sangkhobol and Skerman (1981) with Chitinophaga pinensis UQM 2034T as its type species. The species was isolated from forest litter and showed strong chitinolytic but no cellulolytic activity Communicated by Erko stackebrandt. The GenBank accession numbers for the 16S rRNA gene sequence and the genome sequence of strain H33E-04T are MN998543 and CP048113, respectively. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-020-02066-9) contains supplementary material, which is available to authorized users. * Hang‑Yeon Weon [email protected] 1
Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju‑gun, Jeollabuk‑do 55365, Republic of Korea
(Sangkhobol and Skerman 1981). Kämpfer et al. (2006) reclassified [Flexibacter] sancti, [Flexibacter] filiformis, [Flexibacter] japonensis, and [Cytophaga] arvensicola as members of the genus Chitinophaga (Kämpfer et al. 2006). At the time of writing, the genus Chitinophaga consists of 37 species with validly published and 3 species with invalidly published names (https: //lpsn.dsmz.de/genus/ chitin opha ga). Members of the genus Chitinophaga are us
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