Microbispora cellulosiformans sp. nov., a novel actinomycete with cellulase activity isolated from soil in the cold regi
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ORIGINAL PAPER
Microbispora cellulosiformans sp. nov., a novel actinomycete with cellulase activity isolated from soil in the cold region Xiujie Gong . Wensheng Xiang . Xu Cao . Yang Yu . Yubo Hao . Liang Li . Qiuju Wang . Hongtao Zou . Chunrong Qian
Received: 30 October 2019 / Accepted: 26 September 2020 Ó Springer Nature Switzerland AG 2020
Abstract A novel cellulase-producing actinomycete strain Gxj-6T, isolated from soil in the cold region (Heihe city, Heilongjiang province, the northernmost part of China), subjected to a taxonomic study using a polyphasic approach. In the neighbour-joining phylogenetic tree based on 16S rRNA gene sequences, strain Gxj-6T fell within the clade comprising the type strains of species of the genus Microbispora. 16S rRNA gene sequence similarity studies exhibited that species Gxj6T was most closely related to Microbispora Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10482-020-01477-4) contains supplementary material, which is available to authorized users. X. Gong H. Zou (&) College of Land and Environment, Shenyang Agricultural University, No.120, DongLing Road, Shenhe District, Shenyang 110866, People’s Republic of China e-mail: [email protected] X. Gong Y. Yu Y. Hao L. Li C. Qian (&) Institute of Farming and Cultivation, Heilongjiang Academy of Agricultural Sciences, No. 368 Xuefu Road, Nangang District, Harbin 150086, People’s Republic of China e-mail: [email protected]
bryophytorum NEAU-TX2-2T (99.45%), Microbispora fusca NEAU-HEGS1-5T (99.41%), Microbispora camponoti 2C-HV3T (99.31%) and Microbispora rosea subsp. rosea JCM 3006T (98.68%). Organism Gxj-6T contained MK-9(H2) as the predominant ubiquinone and C18:0 10-methyl as the major fatty acid. The major polar lipids of culture Gxj-6T were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol mannoside, three unidentified phospholipids, two unidentified glycolipids and two unidentified lipids. The DNA G?C content of strain Gxj-6T was 71.25 mol%. The morphological and chemotaxonomic properties of the strain are also consistent with X. Cao Institute of Microbiology, Heilongjiang Academy of Sciences, No. 68 Zhaolin Street, Daoli District, Harbin 150086, People’s Republic of China Q. Wang Institute of Soil Fertilizer and Environment Resources, Heilongjiang Academy of Agricultural Sciences, No. 368 Xuefu Road, Nangang District, Harbin 150086, People’s Republic of China
W. Xiang Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin 150030, People’s Republic of China
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Antonie van Leeuwenhoek
those members of the genus Microbispora. Combinated with the lower DNA–DNA relatedness values, phenotypic properties, physiology and biochemistry distinctiveness with other recognized species strains, revealed that strain Gxj-6T is separated from other phylogenetically closel
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