Mucilaginibacter calamicampi sp. nov., a member of the family Sphingobacteriaceae isolated from soil at a field of reeds
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ORIGINAL PAPER
Mucilaginibacter calamicampi sp. nov., a member of the family Sphingobacteriaceae isolated from soil at a field of reeds Jung-Hoon Yoon • Sooyeon Park Yong-Taek Jung
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Received: 4 February 2013 / Accepted: 8 April 2013 / Published online: 13 April 2013 Ó Springer Science+Business Media Dordrecht 2013
Abstract A Gram-negative, aerobic, non-flagellated, non-gliding, rod-shaped bacterial strain, designated WR-R1YT, was isolated from soil at a field of reeds in South Korea. Strain WR-R1YT grew optimally at 30 °C, at pH 7.0–7.5 and in the absence of NaCl. Neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain WR-R1YT fell within the clade comprising Mucilaginibacter species, coherently clustering with the type strain of Mucilaginibacter composti, with which it exhibited the highest 16S rRNA gene sequence similarity value of 97.6 %. Sequence similarities to the type strains of the other Mucilaginibacter species and the other species used in the phylogenetic analysis were 93.1–96.9 % and \91.1 %, respectively. Strain WR-R1YT contained MK-7 as the predominant menaquinone and isoC15:0, summed feature 3 (C16:1 x6c and/or C16:1 x7c), C16:0 and iso-C17:0 3-OH as the major fatty acids. The major polar lipids were phosphatidylethanolamine and
The GenBank/EMBL/DDBJ accession no. for the 16S rRNA gene sequence of strain WR-R1YT is KC537738.
Electronic supplementary material The online version of this article (doi:10.1007/s10482-013-9922-4) contains supplementary material, which is available to authorized users. J.-H. Yoon (&) S. Park Y.-T. Jung Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea e-mail: [email protected]
one unidentified aminophospholipid. The DNA G?C content of strain WR-R1YT was 43.1 mol% and its mean DNA–DNA relatedness value with M. composti KACC 14956T was 17 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain WR-R1YT is separate from other Mucilaginibacter species. On the basis of the data presented, strain WR-R1YT represents a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter calamicampi sp. nov. is proposed. The type strain is WR-R1YT (= KCTC 32214T = CCUG 63418T). Keywords Mucilaginibacter calamicampi sp. nov. Novel species Polyphasic taxonomy Field of reeds
Introduction The genus Mucilaginibacter, a member of the family Sphingobacteriaceae of the phylum Bacteroidetes, was first proposed by Pankratov et al. (2007) with the description of two novel species, M. paludis (type species) and M. gracilis. Nevertheless, the number of Mucilaginibacter species has increased considerably through subsequent descriptions of novel species. At the time of writing, the genus Mucilaginibacter comprised 21 species with validly published names (Pankratov et al. 2007; Urai et al. 2008; An et al. 2009; Luo et al. 2009; Jeon et al. 2009; Baik et al. 2010;
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Madhaiyan et al. 2010; Ma¨nnisto¨ et al. 2010;
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