Nonomuraea terrae sp. nov., isolated from arid soil
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ORIGINAL PAPER
Nonomuraea terrae sp. nov., isolated from arid soil Hilal Ay1 Received: 14 April 2020 / Revised: 31 May 2020 / Accepted: 4 June 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract During the course of isolating rare actinobacteria from unexplored habitats, strain C H32T was obtained from an arid soil sample in eastern Anatolia, Turkey. Polyphasic characterization and comprehensive genome analyses showed that the strain is a member of the genus Nonomuraea and it is closely related to Nonomuraea gerenzanensis ATCC 39727T, Nonomuraea polychroma DSM 4 3925T and Nonomuraea maritima FXJ7.203T with gene identity level of 98.7%, 98.2% and 98.1%, respectively. The whole-cell hydrolysates contain meso-diaminopimelic acid as diagnostic diaminoacid and glucose, ribose, galactose, mannose and madurose as whole cell sugars. The predominant menaquinones are MK-9(H4), MK-9(H6) and MK-9(H2) while MK-9 exists as minor component. The polar lipid profile consists of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, glycolipid, glycophospholipids, phospholipids and unidentified lipids. The major cellular fatty acids are iso-C16:0 and C17:0 10-methyl. The total genome size is about 9.6 Mb and the G + C content is 71.0%. The genome contains biosynthetic gene clusters encoding for terpenes, siderophores, a type III polyketide synthase, a non-ribosomal polypeptide synthetase and a bacteriocin. The genome-based comparisons of the strain with its phylogenetic neighbours, as indicated by digital DNA–DNA hybridization and average nucleotide identity analyses, reveal that strain CH32T (= JCM 33876T = KCTC 49368T) is a novel member of the genus Nonomuraea, for which Nonomuraea terrae sp. nov. is proposed. Keywords Actinobacteria · Biosynthetic gene clusters · Genome mining · Nonomuraea · Phylogenomics
Introduction Actinobacteria are ecologically and biotechnologically important group of microorganisms due to their high capacity to synthesize a wide range of enzymes and bioactive metabolites (Barka et al. 2016; D’Argenio et al. 2016). Among this group, members of the genus Streptomyces have been widely used as the most prolific source of valuable compounds (Barka et al. 2016). However, recent works have shown that non-Streptomyces genera, such as the members Communicated by Erko Stackebrandt. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-020-01941-9) contains supplementary material, which is available to authorized users. * Hilal Ay [email protected] 1
Department of Molecular Biology and Genetics, Faculty of Science and Arts, Ondokuz Mayis University, Samsun, Turkey
of the family Streptosporangiaceae, have been sources for many novel bioactive compounds (Komaki et al. 2016; Tocchetti et al. 2015; Yushchuk et al. 2020). The genus Nonomuraea is one of the members of the family Streptosporangiaceae (Ay et al. 2020) with distinct metabolic and genomic potential as exemplified by recent works
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