Rhizobium panacihumi sp. nov., an isolate from ginseng-cultivated soil, as a potential plant growth promoting bacterium
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ORIGINAL PAPER
Rhizobium panacihumi sp. nov., an isolate from ginseng-cultivated soil, as a potential plant growth promoting bacterium Jong‑Pyo Kang1 · Yue Huo1 · Yeon‑Ju Kim1 · Jong‑Chan Ahn2 · Joon Hurh2 · Dong‑Uk Yang1 · Deok‑Chun Yang1,2 Received: 10 August 2018 / Revised: 15 September 2018 / Accepted: 19 September 2018 © Springer-Verlag GmbH Germany, part of Springer Nature 2018
Abstract A novel bacterial strain designated D CY116T was isolated from ginseng-cultivated soil in Gochang-gun, Republic of Korea. T Strain DCY116 , belongs to the genus Rhizobium, and is closely related to Rhizobium yantingense H66T (98.3%), Neorhizobium huautlense S02T (98.2%), Rhizobium soli DS-42T (98.1%), Rhizobium smilacinae PTYR-5T (97.9%), and Neorhizobium alkalisoli CCBAU 0 1393T (97.9%) based on 16S rRNA gene sequence analysis. Analysis of the housekeeping genes atpD, recA, and glnII showed low levels of sequence similarity (96.8%) between strain DCY116T and other closely related species. Strain D CY116T was Gram-stain negative, motile by peritrichous flagella, rod-shaped, strictly aerobic, catalase- and oxidase-positive. Q-10 was the predominant ubiquinone. The major cellular fatty acids were identified as C 16:0 and summed feature 8 ( C18:1 ω7c and/or C 18:1 ω6c). The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, and an unknown lipid (L1-3). Genomic DNA G + C content of strain D CY116T was determined to be 57.2 mol%. DNA–DNA T homology values between strain D CY116 and closely related species of the genus Rhizobium were lower than 40%. Strain DCY116T produced indole-3-acetic acid, siderophores, and was able to solubilize phosphate as a potential plant growth promoting bacterium. In conclusion, the results of this study support strain D CY116T as a novel species of the genus Rhizobium, for which the name Rhizobium panacihumi is proposed. The type strain is D CY116T (= KCTC 6 2017T = JCM 32251T). Keywords Rhizobium panacihumi · Ginseng-cultivated soil · Plant growth promoting bacterium
Introduction Communicated by Erko Stackebrandt. The NCBI GenBank accession number for the 16S rRNA, atpD, recA and glnII gene sequence of strain DCY116T is KY996486, MF314825, MF314827, and MF314826. DPD (digital protologue database) Taxon number: TA00436. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-018-1578-z) contains supplementary material, which is available to authorized users. * Yeon‑Ju Kim [email protected] * Deok‑Chun Yang [email protected] 1
Department of Oriental Medicinal Biotechnology, Kyung Hee University, Seocheon‑dong, Giheung‑gu, Yongin, Gyeonggi‑do 17104, Republic of Korea
Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Seocheon‑dong, Giheung‑gu, Yongin, Gyeonggi‑do 17104, Republic of Korea
2
The genus Rhizobium was first described as being comprised of root and/or stem nodule associated bacteria by Frank (1889) and later emended by Young et al. (2001). At the time of
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