Rufibacter radiotolerans sp. nov., a novel gamma-radiation-resistant bacterium isolated from rice field
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ORIGINAL PAPER
Rufibacter radiotolerans sp. nov., a novel gamma‑radiation‑resistant bacterium isolated from rice field Myung Kyum Kim1 · Sathiyaraj Srinivasan1 Received: 9 June 2020 / Revised: 10 August 2020 / Accepted: 2 September 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract A red-pigmented isolate, designated D G31DT, was isolated from the soil of a rice field in South Korea. Cells were strictly aerobic, short rod-shaped, Gram-negative, oxidase-positive, and catalase-positive. The major polar lipid was phosphatidylethanolamine (PE), and the predominant respiratory quinone was MK-7. DG31DT showed high resistance to both the UV and gamma radiations. Based on the 16S rRNA gene sequence, strain D G31DT was related to Rufibacter immobilis MCC P 1T T (97.1%) and Rufibacter tibetensis 1351 (96.5%). The average nucleotide identity (ANI) and in silico DNA–DNA hybridization (isDDH) values between the strain DG31DT and the related Rufibacter sp. were between 74.1–77.6% and 19.9–21.9%, respectively. Strain D G31DT grew between 15 and 30 °C (optimum, 25 °C), at pH 6.0–8.0 (optimum, pH 7.0) and tolerated up to 1.0% NaCl (w/v). The major cellular fatty acids of the novel strain were summed feature 3 (C16:1 ω7c/C16:1 ω6c) and C16:1 ω5c. Flexirubin-type pigments were absent. On the basis of the phenotypic, phylogenetic, and chemotaxonomic data, strain DG31DT represents a novel species of the genus Rufibacter, for which the name Rufibacter radiotolerans sp. nov. is proposed. The type strain is DG31DT (= JCM 19446T = KCTC 32454T). Keywords Cytophagaceae · Rufibacter · Radiation · Taxonomy
Introduction The family Cytophagaceae belongs to the phylum Bacteroidetes and consists of validated bacterial genera. There are several reported gamma and UV radiation-resistant bacterial species in the family Cytophagaceae, belonging to the genus Hymenobacter (Zhang et al. 2007). The UVC Communicated by Erko stackebrandt. The NCBI GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain DG31DT (= JCM 19446T = KCTC 32454T) is KF303587. The draft genome sequence of strain 17J3626T was deposited at DDBJ/EMBL/GenBank under the accession number CP010777. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-020-02033-4) contains supplementary material, which is available to authorized users. * Sathiyaraj Srinivasan [email protected] 1
radiation-resistant strain belonging to the genus Rufibacter was also identified (Abaydulla et al. 2012). At the time of writing the genus, Rufibacter contained the following 35 validly named species (https://lpsn.dsmz.de/genus/rufibacter). Rufibacter strains can be easily differentiated from Nibribacter by their gliding motility and the absence of 3OHC15:0 fatty acids. The isolated strain D G31DT showed the characteristic survival curve for gamma-radiation resistance, similar to Deinococcus radiodurans R1 (Srinivasan et al. 2015). The radiation-resistant microorganism contains sophistica
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