Sphingomonas hominis sp. nov., isolated from hair of a 21-year-old girl
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ORIGINAL PAPER
Sphingomonas hominis sp. nov., isolated from hair of a 21-year-old girl Dao-Feng Zhang Wen-Jun Li
. Xi-Wen Cui . Zhe Zhao . Ai-Hua Zhang . Jian-Ke Huang .
Received: 29 June 2020 / Accepted: 4 August 2020 Ó Springer Nature Switzerland AG 2020
Abstract A Gram-stain-negative, aerobic, motile strain, HHU CXWT, was isolated from hair of a healthy 21-year-old female student of Hohai University, Nanjing, China. The 16S rRNA gene sequence analysis indicated that HHU CXWT represents a member of the genus Sphingomonas with the highest sequence similarity (97.6%) to the type strain S. aquatilis JSS7T. HHU CXWT grew at 4–35 °C and pH
Dao-Feng Zhang and Xi-Wen Cui contributed equally to this work.
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10482-020-01460-z) contains supplementary material, which is available to authorized users. D.-F. Zhang (&) X.-W. Cui Z. Zhao A.-H. Zhang J.-K. Huang Institute of Marine Biology, College of Oceanography, Hohai University, Nanjing, People’s Republic of China e-mail: [email protected]
6–8, with optimum growth at 28 °C and pH 7. Tolerance to NaCl was up to 2% (w/v) with optimum growth in 0.5–1.0% NaCl. The major fatty acids were C16:0, C17:1x6c, C18:1x7c11-methyl, summed feature 3 (C16:1x7c and/or C16:1x6c), and summed feature 8 (C18:1x7c and/or C18:1x6c). The predominant isoprenoid quinone was ubiquinone-10. The polar lipids were diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), sphingoglycolipid (SGL), phosphatidylinositol mannosides (PIM), and an unidentified glycolipid (GL). The DNA G ? C content was 67.1%. The average nucleotide identity (ANI) values and digital DNA–DNA hybridization (dDDH) between HHU CXWT and closely related members of the genus
W.-J. Li State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, People’s Republic of China
W.-J. Li (&) State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, People’s Republic of China e-mail: [email protected]
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Antonie van Leeuwenhoek
Sphingomonas were all below the cut-off level (95–96% and 70%, respectively) for species delineation. On the basis of the phenotypic, phylogenetic and chemotaxonomic characterizations, HHU CXWT represents a novel species of the genus Sphingomonas, for which the name Sphingomonas hominis sp. nov. is proposed. The type strain is HHU CXWT (= KCTC 72946T = CGMCC 1.17504T = MCCC 1K04223T). Keywords Sphingomonas hominis sp. nov Sphingoglycolipid bac120 tree
Materials and methods Isolation of the bacterial strain and culture conditions The hair was collected from a female student in class, and was cut into pieces (\ 1 cm in length) before being bespattered on the trypticase soy agar (TSA) medium (BD Diagnostics, Maryland, USA). The plate was incubated a
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