Streptomyces buecherae sp. nov., an actinomycete isolated from multiple bat species
- PDF / 1,253,110 Bytes
- 9 Pages / 547.087 x 737.008 pts Page_size
- 65 Downloads / 159 Views
(0123456789().,-volV) ( 01234567 89().,-volV)
ORIGINAL PAPER
Streptomyces buecherae sp. nov., an actinomycete isolated from multiple bat species Paris S. Hamm . Christopher A. Dunlap . Michael W. Mullowney . Nicole A. Caimi . Neil L. Kelleher . Regan J. Thomson . Andrea Porras-Alfaro . Diana E. Northup
Received: 22 September 2020 / Accepted: 22 October 2020 Ó Springer Nature Switzerland AG 2020
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10482-020-01493-4) contains supplementary material, which is available to authorized users.
B-24617T), and Streptomyces yeochonensis (NRRL B-24245T). A complete genome of the type strain was assembled to determine its taxonomy and secondary metabolite potential. ANI comparisons between all closely related types strains are shown to be well below the 95–96% species delineation. DNA-DNA relatedness between AC541T and its nearest neighbors ranged between 23.7 and 24.1% confirming novelty. Approximately 1.49 Mb or 17.76% of the whole genome is devoted to natural product biosynthesis. The DNA G ? C content of the genomic DNA of the type strain is 73.13 mol %. Micromorphology depicts ovoid spores with smooth surfaces in flexuous chains. Strains presented an ivory to yellow hue on most ISP media except inorganic salts-starch agar (ISP4) and can grow on D-glucose, mannitol, and D-fructose, but exhibited little to no growth on L-arabinose, sucrose, D-xylose, inositol, L-rhamnose, D-raffinose, and cellulose. This clade possesses the capability to grow from 10 to 45 °C and 12.5% (w/v) NaCl. There was strain
P. S. Hamm Department of Biological Sciences, Western Illinois University, Macomb, IL 61455, USA
M. W. Mullowney N. L. Kelleher R. J. Thomson Department of Chemistry, Northwestern University, Evanston, IL 60208, USA
P. S. Hamm (&) N. A. Caimi D. E. Northup Department of Biology, University of New Mexico, Albuquerque, NM 87131, USA e-mail: [email protected]
A. Porras-Alfaro Institute for Environmental Studies, Western Illinois University, Macomb, IL 61455, USA
C. A. Dunlap U. S. Department of Agriculture, Peoria, IL 61604, USA
Present Address: A. Porras-Alfaro Division of Environmental Biology, National Science Foundation, Alexandria, VA 22314, USA
Abstract A putative novel clade within the genus Streptomyces was discovered following antifungal screening against Pseudogymnoascus destructans, the causative agent of white-nose syndrome, and described using multi-locus sequencing analysis. Swabs from both the cave myotis bat (Myotis velifer) and the Brazilian free-tailed bat (Tadarida brasiliensis) in southern New Mexico bore isolates AC536, AC541T and AC563, which were characterised using phylogenetic, morphological, and phenotypic analyses. Multi-locus sequence analysis positions AC541T with neighbors Streptomyces rubidus (NRRL B-24619T), Streptomyces guanduensis (NRRL The GenBank accession number for complete genome of Streptomyces buecherae AC541T is CP060404.
123
Antonie van Leeuwenhoek
growth variation in pH, but all
Data Loading...
