Stenotrophomonas cyclobalanopsidis sp. nov., isolated from the leaf spot disease of Cyclobalanopsis patelliformis
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ORIGINAL PAPER
Stenotrophomonas cyclobalanopsidis sp. nov., isolated from the leaf spot disease of Cyclobalanopsis patelliformis Dan-ran Bian . Han Xue . Chun-gen Piao . Yong Li
Received: 28 April 2020 / Accepted: 22 July 2020 Ó Springer Nature Switzerland AG 2020
Abstract A Gram-negative, facultatively anaerobic, motile bacterial strain, TPQG1-4T, was isolated from the leaf of Cyclobalanopsis patelliformis with spot disease. The isolate was investigated using the polyphasic taxonomic approach. 16S rRNA gene sequencing and analyzing revealed that the novel strain shares the highest sequence similarity with Stenotrophomonas lactitubi M15T (99.6%), Stenotrophomonas indicatrix WS40T (99.4%), Stenotrophomonas maltophilia IAM 12423T (99.2%) and Stenotrophomonas pavanii LMG 25348T (99.0%). In phylogenetic trees based on 16S rRNA gene sequences, the novel strain branched independently from other species of Stenotrophomonas. Average nucleotide identity values between the novel isolate and S. lactitubi M15T, S. indicatrix WS40T, S. maltophilia IAM 12423T, S. pavanii LMG 25348T, and Pseudomonas geniculata ATCC 19374T were 87.2%, 87.3%, 86.3%, 88.0%, and 81.3%, respectively, suggesting the isolate was a novel species of the
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10482-020-01453-y) contains supplementary material, which is available to authorized users. D. Bian H. Xue C. Piao Y. Li (&) The Key Laboratory of National Forestry and Grassland Administration on Forest Protection, Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing, China e-mail: [email protected]
genus Stenotrophomonas. The DNA G ? C content of TPQG1-4T is 67.1 mol%. The major fatty acids were iso-C15:0 (25.4%) and anteiso-C15:0 (17.0%). The polar lipids of TPQG1-4T included phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, amino phospholipid and phospholipid. Based on phenotypic and genotypic characteristics, the strain represents a novel species in the genus Stenotrophomonas, for which the name Stenotrophomonas cyclobalanopsidis sp. nov. is proposed. The type strain is TPQG1-4T (= CFCC 15341T= LMG 31208T). Keywords Stenotrophomonas Polyphasic taxonomic Average nucleotide identity Genome
Introduction The genus Stenotrophomonas was established in 1993 to reclassify Xanthomonas maltophilia (formerly Pseudomonas maltophilia) as Stenotrophomonas maltophilia (Palleroni and Bradbury 1993). At the time of writing in 2020, the genus Stenotrophomonas comprises 18 species with validly published names isolated from a large range of natural and artificial environments and geographical regions, including the latest species S. bentonitica (Sa´nchez-Castro et al. 2017), S. indicatrix (Weber et al.2018), S. lactitubi
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Antonie van Leeuwenhoek
(Weber et al.2018), S. pictorum (Ouattara et al. 2017), S. tumulicola (Handa et al. 2016) and the type species S. maltophilia LMG 958T (Paller
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