Xylophilus rhododendri sp. nov., Isolated from Flower of Royal Azalea, Rhododendron schlippenbachii
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Xylophilus rhododendri sp. nov., Isolated from Flower of Royal Azalea, Rhododendron schlippenbachii Shin Ae Lee1 · Jun Heo1 · Tae‑Wan Kim1 · Mee‑Kyung Sang1 · Jaekyeong Song1 · Soon‑Wo Kwon1 · Hang‑Yeon Weon1 Received: 3 May 2020 / Accepted: 1 September 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract A bacterial strain, designated CJ1-R5T, was isolated from the flower of the royal azalea plant (Rhododendron schlippenbachii) collected in Jeju Island, Republic of Korea. The strain was a Gram-negative, strictly aerobic, motile, rod-shaped bacterium, growing at a temperature range of 4–33 °C (optimum 28–30 °C), pH 5.0–9.0 (optimum pH 7.0–8.0), and 0–1% NaCl (optimum 0%). The 16S rRNA sequence analysis of strain CJ1-R5T revealed the highest sequence similarity (97.9%) with Xylophilus ampelinus ATCC 33914T, and sequence similarities of less than 97.2% with other validly named species. Phylogenetic tree analysis based on the 16S rRNA gene sequences showed that strain CJ1-R5T clustered with Xylophilus ampelinus ATCC 33914T and two uncultured bacterial clones. The only quinone observed in strain CJ1-R5T was ubiquinone-8. The polar lipids observed were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminophospholipid and two unidentified lipids. The major fatty acids were C 16:0, C17:0 cyclo, and summed feature 8 ( C18:1 ω7c and/or C18:1 ω6c). The genome size of strain CJ1-R5T was 5.85 Mbp. The genomic G + C content was 68.4 mol%. ANI and dDDH values between strain CJ1-R5T and Xylophilus ampelinus ATCC 3 3914T were 79.0% and 22.5%, respectively. Based on the polyphasic taxonomic data, strain CJ1-R5T is considered to represent a novel species, for which the name Xylophilus rhododendri sp. nov. is proposed. The type strain is CJ1-R5T (= KACC 21265T = CCTCC AB2020030T)
Introduction The genus Xylophilus was proposed by Willems et al. when reclassifying Xanthomonas ampelina [1]. Xanthomonas ampelina was originally described as a plant pathogen causing bacterial necrosis and canker of grape vines; this leads to large economic losses through the decaying of newly developed vines or the whole plant in the Mediterranean region and South Africa [2, 3]. DNA-rRNA hybridization, The GenBank number for the 16S rRNA gene sequence, plasmid sequence and genome sequence of strain CJ1-R5T are MN912104, CP047651 and CP047650, respectively. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00284-020-02194-x) contains supplementary material, which is available to authorized users. * Hang‑Yeon Weon [email protected] 1
Agricultural Microbiology Division, Rural Development Administration, National Institute of Agricultural Sciences, Wanju‑gun, Jeollabuk‑do 55365, Republic of Korea
electrophoresis of cellular proteins, and enzymatic features showed that Xanthomonas ampelina was a separate species from the other members of Xanthomonas. Therefore, the species was transferred to a new genus, Xylophilus, classified int
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