Bradyrhizobium campsiandrae sp. nov., a nitrogen-fixing bacterial strain isolated from a native leguminous tree from the
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ORIGINAL PAPER
Bradyrhizobium campsiandrae sp. nov., a nitrogen‑fixing bacterial strain isolated from a native leguminous tree from the Amazon adapted to flooded conditions Daniele Cabral Michel1 · Elaine Martins da Costa2 · Amanda Azarias Guimarães1 · Teotonio Soares de Carvalho1 · Polyane Santos de Castro Caputo1 · Anne Willems3 · Fatima Maria de Souza Moreira1 Received: 20 May 2020 / Revised: 23 July 2020 / Accepted: 13 August 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract The nitrogen-fixing bacterial strain UFLA 01-1174T was isolated from nodules of Campsiandra laurilifolia Benth. originating from the Amazon region, Brazil. Its taxonomic position was defined using a polyphasic approach. Analysis of the 16S rRNA gene placed the strain in the Bradyrhizobium genus, the closest species being B. guangdongense CCBAU 5 1649T and B. guangzhouense CCBAU 5 1670T, both with 99.8% similarity. Multilocus sequence analysis (MLSA) of recA, gyrB, glnII, rpoB, atpD, and dnaK indicated that UFLA 01-1174T is a new species, most closely related to B. stylosanthis BR 4 46T (94.4%) and B. manausense BR 3351T (93.7%). Average nucleotide identity (ANI) differentiated UFLA 01-1174T from the closest species with values lower than 90%. The G + C content in the DNA of UFLA 01-1174T is 63.6 mol%. Based on this data, we conclude that the strain represents a new species. The name proposed is Bradyrhizobium campsiandrae, with UFLA 01-1174T (= INPA 394BT = LMG 10099T) as type strain. Keywords Nitrogen fixation · Leguminous tree · Bradyrhizobium · Taxonomy
Introduction Biological nitrogen fixation is essential for ecosystem functioning. This process, mediated by some prokaryote species, is responsible for converting atmospheric N 2 into Communicated by Erko Stackebrandt. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-020-02022-7) contains supplementary material, which is available to authorized users. * Fatima Maria de Souza Moreira [email protected] 1
Setor de Biologia, Microbiologia e Processos Biológicos Do Solo, Departamento de Ciência Do Solo, Universidade Federal de Lavras, Campus UFLA, Lavras, Minas Gerais 37200‑900, Brazil
2
Universidade Federal Do Piauí, Campus Professora Cinobelina Elvas, Bom Jesus, Piauí 64900‑000, Brazil
3
Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, K.L. Ledeganckstraat 35, 9000 Ghent, Belgium
plant-assimilable N. The rhizobial group, composed of bacteria that establish a symbiotic relationship with legume species, are recognized as efficient biological nitrogen fixers. The Bradyrhizobium genus, belonging to this group, is known to be a predominant symbiont of most nodulating legumes and can interact with a large group of legume clades (Moreira et al. 1993; Parker 2015). Bradyrhizobium species can establish symbiosis and fix N2 with early and later emerging legume clades in subfamilies, such as Papilionoideae and Caesalpinioideae of the Legumino
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