Luteimonas wenzhouensis Sp. Nov., A Chitinolytic Bacterium Isolated from a Landfill Soil
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Luteimonas wenzhouensis Sp. Nov., A Chitinolytic Bacterium Isolated from a Landfill Soil Jie Zhou1 · Jianhao Chen1 · Jieyu Ma1 · Ning Xu1 · Fengxue Xin1,2 · Wenming Zhang1,2 · Hao Zhang3 · Weiliang Dong1,2 · Min Jiang1,2 Received: 16 May 2020 / Accepted: 11 November 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract A Gram-staining-negative, aerobic, non-motile, rod-shaped bacterium with degradation ability of chitin, designated strain YD-1 T, was isolated from landfill soil sample collected in Wenzhou, Zhejiang province, China. The growth of strain YD-1 T occurred optimally in the tryptone soy broth (TSB) with 1.0% NaCl at pH 7.0–8.0, 30 °C. Ubiquinone-8 (Q-8) was the predominant quinone. The polar lipids of strain YD-1 T consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, five glycolipids and four lipids. The major fatty acids were iso-C 15:0 (30.7%), iso-C17:1ω9c (23.2%), iso-C11:0 (18.9%), iso-C11:0 3-OH (6.8%) and iso-C17:0 (5.9%). Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain YD-1 T was affiliated to the genus Luteimonas with the highest similarity to Luteimonas marina KCTC 1 2327 T (97.3%), followed by Luteimonas aquatica DSM 2 2088 T (96.5%) and Luteimonas composti CCUG 5 3595 T (96.4%). The genomic DNA G + C content of strain YD-1 T was 71.8 mol%. Average nucleotide identity (ANI) and the digital DNA–DNA hybridizations (dDDH) for draft genomes between strain YD-1 T and Luteimonas marina KCTC 1 2327 T were 82.7% and 26.1%, respectively. On the basis of genotypic, phenotypic and chemotaxonomic data, strain YD-1 T is considered to represent a novel species to degrade chitin in the genus Luteimonas, for which the name Luteimonas wenzhouensis sp. nov. is proposed, with YD-1 T (= KCTC 7 2425 T = CCTCC AB 2019153 T) as the type strain.
Introduction The genus Luteimonas was first proposed by Finkmann et al. with one type species Luteimonas mephitis [1]. The genus is a member of the family Xanthomonadaceae, class Gammaproteobacteria and closely to the genera Lysobacter, Xanthomonas, Pseudoxanthomonas and Stenotrophomonas. The Electronic Supplementary Material The online version of this article (https://doi.org/10.1007/s00284-020-02293-9) contains supplementary material, which is available to authorized users. * Weiliang Dong [email protected] 1
State Key Laboratory of Materials‑Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, P.R. China
2
Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University, Nanjing 211800, P.R. China
3
School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, P.R. China
species within the genus Luteimonas share some characteristics such as Gram-stain-negative, rod-shaped, yellow coloured and the presence of ubiquinone-8 (Q-8) as the major ubiquinone and iso-C15:0 as the predominant fatty acid. At the t
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