Reinekea thalattae sp. nov., a New Species of the Genus Reinekea Isolated from Surface Seawater in Sehwa Beach
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Reinekea thalattae sp. nov., a New Species of the Genus Reinekea Isolated from Surface Seawater in Sehwa Beach Inhyup Kim1 · Geeta Chhetri1 · Jiyoun Kim1 · Minchung Kang1 · Taegun Seo1 Received: 14 May 2020 / Accepted: 22 September 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract A Gram-stain-negative, non-pigmented, curved rod-shaped, single polarly flagellated, facultatively anaerobic bacterium, designated as S SH23T, was isolated from surface seawater sample collected at the Sehwa Beach in South Korea. The novel isolate required NaCl for growth and grew optimally between 2 and 3% NaCl. Strain SSH23T showed high 16S rRNA gene sequence similarities with Reinekea marinisedimentorum DSM 1 5388T (96.4%), Reinekea marina KACC 1 7315T (96.2%), Reinekea blandensis KACC 1 7315T (95.9%) and Reinekea aestuarii KCTC 2 2813T (95.6%). The major polar lipids of strain SSH23T were phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The major cellular fatty acids of strain SSH23T were C16:0, summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), and summed feature 8 (C18:1 ω7c and/or C18:1 ω6c). The predominant respiratory quinone was found to be ubiquinone-8. The average nucleotide identity values of strain SSH23T with R. marinisedimentorum DSM 15388T and R. blandensis MED297T were determined to be 72.2% and 69.8%, respectively. The G+C content of the genomic DNA was 45.5 mol%. Based on genotypic, phenotypic, chemotaxonomic, and phylogenetic analyses, strain SSH23T was considered to represent a novel member of the genus Reinekea, for which the name Reinekea thalattae sp. nov. is proposed. The type strain of Reinekea thalattae is SSH23T (= KACC 21168T = NBRC 113795T).
Introduction The genus Reinekea, a member of the family Saccharospirillaceae in the phylum Proteobacteria was first proposed by Romanenko [1] and the genus description was later emended by Kang [2]. According to the LPSN (https://lpsn.dsmz.de/ genus/reinekea) [3], currently four species of the genus have been isolated from different sources such as seawater [4], coastal marine sediment [1], tidal flat sediment [5], and surface seawater [2]. Growth occurs at 4–42 °C [2]. In the widely accepted definition of Kushner and Kamekura [6] it would be a genus of slightly halophilic bacteria, some of them displaying tolerance up to 10% NaCl [2]. The DNA G+C content ranges from 45.5–52.4 mol%. The present study is a continuation of our previous studies that
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00284-020-02224-8) contains supplementary material, which is available to authorized users. * Taegun Seo [email protected] 1
Department of Life Science, Dongguk University-Seoul, Goyang 10326, South Korea
investigated bacterial diversity and bioprospecting in different regions of South Korea. This study involved the characterization and classification of a novel Gram-stain-negative Reinekea strain, designated as S SH23T, which was isolated from
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