Roseovarius lacus sp. nov., isolated from Yuncheng Saline Lake, China
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ORIGINAL PAPER
Roseovarius lacus sp. nov., isolated from Yuncheng Saline Lake, China Jiafeng Pan1,2 · Zhen Yu2 · Jia Tang2 · Guiqin Yang2 · Li Zhuang2 · Zhi Liu1 · Shungui Zhou3
Received: 22 July 2016 / Revised: 22 August 2016 / Accepted: 20 September 2016 © Springer-Verlag Berlin Heidelberg 2016
Abstract Strain GSS12T, a Gram-negative, aerobic, nonflagellated, ovoid- to rod-shaped (0.5–0.7 × 0.9–3.0 µm) bacterium, was isolated from Yuncheng Saline Lake, China. Growth occurred with 0.5–16.0 % (w/v) NaCl (optimum 4.5 %), at pH 5.0–10.0 (optimum pH 6.0–6.5) and at 10–50 °C (optimum 37 °C). The major fatty acids (>5.0 %) found in GSS12T were summed feature 8 (72.2 %), C16:0 (9.0 %) and C18:1 ω7c 11-methyl (6.4 %). The DNA G+C content was 62.7 mol%. Analysis of the 16S rRNA gene sequences showed that strain GSS12T forms a stable clade with species of the genus Roseovarius, being related to R. pacificus 81-2T and R. litoreus GSW-M15T with 97.9 and 96.7 % of sequence similarity, respectively.
The DNA–DNA relatedness values between strain GSS12T and R. pacificus 81-2T and R. halotolerans HJ50T were low (36 and 29 %, respectively). The phenotypic, physiological, biochemical and genetic characteristics support the assignment of strain GSS12T to the genus Roseovarius and represent a novel species. The name Roseovarius lacus sp. nov. is proposed, with strain GSS12T (=KCTC 52185T =MCCC 1K02302T) as the type strain. Keywords Roseovarius lacus sp. nov. · Saline Lake · Polyphasic taxonomy
Introduction
Communicated by Erko Stackebrandt. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequences of strain GSS12T is KX417301. Electronic supplementary material The online version of this article (doi:10.1007/s00203-016-1296-3) contains supplementary material, which is available to authorized users. * Zhen Yu [email protected] 1
College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, People’s Republic of China
2
Guangdong Key Laboratory of Agricultural Environment Pollution Integrated Control, Guangdong Institute of EcoEnvironmental and Soil Sciences, Guangzhou 510650, People’s Republic of China
3
College of Resources and Environmental Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, People’s Republic of China
The genus Roseovarius, which mostly contains Gramnegative, oxidase- and catalase-positive members (Oh et al. 2009; Rajasabapathy et al. 2014), was established by Labrenz et al. (1999) with the description of R. tolerans. Currently the genus contains 16 species with validly published names, most of which were retrieved from marine environments such as seawater, deep-sea sediment, tidal flats and decayed whale bones (Oh et al. 2009; Wang et al. 2009; Choi et al. 2013; Deng et al. 2015). Members of the genus Roseovarius contained Q-10 as the predominant isoprenoid quinone and C18:1 ω6c and/or C18:1 ω7c as the dominant cellular fatty acids. The DNA G+C content of this genus was in the range of 58.4–68.1 mol% (Deng et al. 2015). In this study, we
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