The complete mitochondrial genome of Odorrana hainanensis (Anura: Ranidae)
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TECHNICAL NOTE
The complete mitochondrial genome of Odorrana hainanensis (Anura: Ranidae) Yongjie Huang1 · Wei Zhao2 · Xinkang Bao2 · Jiabin Lu3 · Fengtang Wang3 · Dandan Liu1,4 · Yinghua Lin1
Received: 18 May 2017 / Accepted: 19 May 2017 © Springer Science+Business Media Dordrecht 2017
Abstract The Chinese Hainan odorous frog, Odorrana hainanensis, is an IUCN red-listed vulnerable species found primarily in forests in Hainan, China. In this study, we firstly report the complete mitochondrial genome of O. hainanensis, which was 17986 bp in length, containing 13 protein-coding genes, 22 transfer RNAs (tRNAs), two rRNA genes, and a putative control region. Phylogenetic analysis based on the concatenated data set of two rRNAs and 13 protein-coding genes indicated that the O. hainanensis was clustered with O. schmackeri. This work will provide a set of useful data on further molecular evolution studies of this endangered species. Keywords Odorrana hainanensis · Mitochondrial genome · Protein-coding genes The Chinese Hainan odorous frog (Odorrana hainanensis) is an anuran species endemic to the Guangxi Zhuang Autonomous Region and Hainan Island in China (Fei et al. 2012). Its primary habitat is 10–20 m wide streams and * Xinkang Bao [email protected] * Yinghua Lin [email protected] 1
Institute of Wetland Research, Chinese Academy of Forestry, Beijing 100091, People’s Republic of China
2
School of Life Science, Lanzhou University, Lanzhou 730000, People’s Republic of China
3
College of Tropical Biology and Agronomy, Hainan Tropical Ocean University, Sanya 572000, People’s Republic of China
4
School of Life Science, Jilin Normal University, Siping 136000, People’s Republic of China
rivulets in forests at altitudes of 200–780 m. The IUCN red list of threatened species (http://www.iucnredlist.org) lists it as a vulnerable species because of its declining populations and the destruction of its natural habitat. Thus, it has become a protected species in nature reserves. Unfortunately, effective protection measures remain unavailable because little is known about this species, particularly the genetic divergence among populations. For better study and conservation of this species, herein, we first determined and characterized the complete mitochondrial (mt) genome of the O. hainanensis. The toe tissue of O. hainanensis was clipped before it was released from Wuzhishan Mountain, Wuzhishan, Hainan, China, and preserved in 95% ethanol. The genomic DNA was extracted following the instructions in the TIANamp Genomic DNA Kit (Tiangen, Beijing, China). The complete mt genome was amplified with nine primer pairs designed. The mt sequences were assembled using the program SeqMan (DNASTAR v.7.10). The organization map of the complete mt genome was generated using the Mtv online tool (http://pacosy.informatik.uni-leipzig.de/mtviz/ mtviz). The complete mt genome of O. hainanensis was 17,986 bp in length (GenBank accession KX056491) and consisted of 13 protein-coding genes, 2 ribosomal RNA (rRNA) genes, 22 tRNAs, and a c
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